Respiration accumulates Calvin cycle intermediates for the rapid start of photosynthesis in Synechocystis sp. PCC 6803

• Published in: Bioscience, biotechnology, and biochemistry Vol. 78; no. 12; pp. 1997 - 2007
• Main Authors: Shimakawa, Ginga, Hasunuma, Tomohisa, Kondo, Akihiko, Matsuda, Mami, Makino, Amane, Miyake, Chikahiro
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.12.2014; Oxford University Press
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; biotechnology; Calvin cycle; carbon; cell respiration; cyanobacteria; Dibromothymoquinone - pharmacology; Gene Expression; glucose; Glucose - metabolism; glycogen; Glycogen - biosynthesis; glycogen phosphorylase; Glycogen Phosphorylase - deficiency; Glycogen Phosphorylase - genetics; mutants; Mutation; Oxidation-Reduction; Oxygen - metabolism; phosphorylase; Photoperiod; photosynthesis; Photosynthesis - drug effects; Photosynthesis - genetics; respiration; ribulose 1,5-diphosphate; Ribulosephosphates - biosynthesis; Synechocystis - drug effects; Synechocystis - genetics; Synechocystis - metabolism; respiration; photosynthesis; cyanobacteria; glycogen phosphorylase
• DOI: 10.1080/09168451.2014.943648
• ISSN: 0916-8451; 1347-6947

We tested the hypothesis that inducing photosynthesis in cyanobacteria requires respiration. A mutant deficient in glycogen phosphorylase (∆GlgP) was prepared in Synechocystis sp. PCC 6803 to suppress respiration. The accumulated glycogen in ΔGlgP was 250-450% of that accumulated in wild type (WT). The rate of dark respiration in ΔGlgP was 25% of that in WT. In the dark, P700 + reduction was suppressed in ΔGlgP, and the rate corresponded to that in (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone)-treated WT, supporting a lower respiration rate in ∆GlgP. Photosynthetic O 2 -evolution rate reached a steady-state value much slower in ∆GlgP than in WT. This retardation was solved by addition of d-glucose. Furthermore, we found that the contents of Calvin cycle intermediates in ∆GlgP were lower than those in WT under dark conditions. These observations indicated that respiration provided the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis. Respiration provides the carbon source for regeneration of ribulose 1,5-bisphosphate in order to drive the rapid start of photosynthesis.