Schistosoma mansoni host-exposed surface antigens characterized by sera and recombinant antibodies from schistosomiasis-resistant rats

• Published in: International journal for parasitology Vol. 40; no. 12; pp. 1407 - 1417
• Main Authors: Sepulveda, Jorge, Tremblay, Jacqueline M., DeGnore, Jon P., Skelly, Patrick J., Shoemaker, Charles B.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.10.2010; [Oxford; New York]: Elsevier Science; Elsevier
• Subjects: Animals; Antibodies, Helminth - genetics; Antibodies, Helminth - immunology; Antigen; Antigens, Helminth - genetics; Antigens, Helminth - immunology; Biological and medical sciences; Female; Fundamental and applied biological sciences. Psychology; Host-Parasite Interactions; Humans; Invertebrates; Life cycle. Host-agent relationship. Pathogenesis; Lung - immunology; Lung - parasitology; Male; Mice; Molecular Sequence Data; Nemathelminthia. Plathelmintha; Protozoa; Rat; Rats; Rats, Inbred F344; scFv; Schistosoma mansoni; Schistosoma mansoni - genetics; Schistosoma mansoni - growth & development; Schistosoma mansoni - immunology; Schistosome; Schistosomiasis mansoni - immunology; Schistosomiasis mansoni - parasitology; Schistosomula; Tegument; Antigen; Tegument; Schistosomula; Rat; scFv; Schistosome; Schistosoma mansoni; Antibody; Rodentia; Trematode disease; Plathelmintha; Parasite; Host; Parasitosis; Helminthiasis; Trematoda; Infection; Schistosomiasis; Vertebrata; Mammalia; Helmintha; Invertebrata

Antibodies from Schistosoma mansoni-infected rats, unlike mice, show a higher titer for schistosome apical tegumental antigens compared with non-apical membrane antigens. These antibodies bind to the surface of living lung-stage worms and to formaldehyde-fixed adult worms. We produced a single-chain...