Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells

• Published in: Nature structural & molecular biology Vol. 20; no. 9; pp. 1131 - 1139
• Main Authors: Yan, Liying, Yang, Mingyu, Guo, Hongshan, Yang, Lu, Wu, Jun, Li, Rong, Liu, Ping, Lian, Ying, Zheng, Xiaoying, Yan, Jie, Huang, Jin, Li, Ming, Wu, Xinglong, Wen, Lu, Lao, Kaiqin, Li, Ruiqiang, Qiao, Jie, Tang, Fuchou
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.09.2013; Nature Publishing Group
• Subjects: 631/136/2086; 631/136/532/2117; 631/1647/514/1949; 631/337/384/2568; Alternative Splicing; Analysis; Biochemistry; Biological Microscopy; Blastocyst - cytology; Blastocyst - metabolism; Blastomeres - cytology; Blastomeres - metabolism; Developmental stages; DNA microarrays; Embryo Culture Techniques; Embryology; Embryonic growth stage; Embryonic stem cells; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Embryos; Female; Gene expression; Gene Expression Profiling; Genetic aspects; Germ Layers - cytology; Germ Layers - metabolism; Humans; Life Sciences; Membrane Biology; Methods; Molecular biology; Oocytes - metabolism; Physiological aspects; Pluripotent Stem Cells - cytology; Pluripotent Stem Cells - metabolism; Protein Structure; resource; Ribonucleic acid; RNA; RNA sequencing; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; Sequence Analysis, RNA; Single-Cell Analysis; Stem cells; Transcriptome; China

Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Single-cell RNA sequencing (RNA-Seq) analysis of 124 individual cells from human preimplantation embryos and embryonic stem cells (hESCs) now provides a com...