Generation and characterization of CRISPR/Cas9-mediated MEN1 knockout BON1 cells: a human pancreatic neuroendocrine cell line

• Published in: Scientific reports Vol. 10; no. 1; p. 14572
• Main Authors: Monazzam, Azita, Li, Su-Chen, Wargelius, Hanna, Razmara, Masoud, Bajic, Duska, Mi, Jia, Bergquist, Jonas, Crona, Joakim, Skogseid, Britt
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 03.09.2020; Nature Publishing Group
• Subjects: 631/67; 631/80; Apoptosis; Biomarkers, Tumor - metabolism; CRISPR-Cas Systems; CYP1A2 protein; Cytochrome; Cytochrome P450; DNA adducts; DNA microarrays; DNA repair; Gene Editing; Gene expression; Gene Expression Regulation, Neoplastic; Humanities and Social Sciences; Humans; Hyperoxia; Inactivation; Lungs; multidisciplinary; Mutation; Neuroendocrine tumors; Neuroendocrine Tumors - metabolism; Neuroendocrine Tumors - pathology; Pancreatic cancer; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Proteome - analysis; Proto-Oncogene Proteins - antagonists & inhibitors; Proto-Oncogene Proteins - genetics; Respiratory distress syndrome; Respiratory therapy; Science; Science (multidisciplinary); Tumor Cells, Cultured

Among patients with the rare diagnosis of pancreatic neuroendocrine tumor (P-NET), a substantial proportion suffer from the inherited cancer syndrome multiple endocrine neoplasia type 1 (MEN1), which is caused by germline mutations of the MEN1 suppressor gene. Somatic mutations and loss of the MEN1...