Adenoviral gene transfer of a u-PA receptor-binding plasmin inhibitor and green fluorescent protein: inhibition of migration and visualization of expression

• Published in: Thrombosis and haemostasis Vol. 84; no. 3; p. 460
• Main Authors: Lamfers, M L, Wijnberg, M J, Grimbergen, J M, Huisman, L G, Aalders, M C, Cohen, F N, Verheijen, J H, van Hinsbergh, V W, Quax, P H
• Format: Journal Article
• Language: English
• Published: Germany 01.09.2000
• Subjects: Adenoviridae - genetics; Animals; Antifibrinolytic Agents - metabolism; Cattle; Cell Division - drug effects; Cell Movement - drug effects; Encephalomyocarditis virus - genetics; Fibrinolysin - antagonists & inhibitors; Fibrinolysin - metabolism; Fibrinolytic Agents - metabolism; Gene Transfer Techniques; Genetic Vectors; Green Fluorescent Proteins; Humans; Indicators and Reagents; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Membrane Proteins - metabolism; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - metabolism; Muscle, Smooth, Vascular - secretion; Pancreas; Plasminogen Activators - metabolism; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Recombinant Fusion Proteins - secretion; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Recombinant Proteins - secretion; Saphenous Vein - cytology; Saphenous Vein - metabolism; Saphenous Vein - secretion; Trypsin Inhibitors - genetics; Trypsin Inhibitors - pharmacology; Trypsin Inhibitors - secretion; Urokinase-Type Plasminogen Activator - genetics; Urokinase-Type Plasminogen Activator - metabolism

Smooth muscle cell migration plays a role in the development of intimal hyperplasia. Given the established role of the plasminogen activation system in cell migration, an approach to therapy is to overexpress an inhibitor of plasmin. Therefore, an adenoviral vector was constructed encoding the hybri...