Differential regulation of cytosolic and peroxisomal bile acid amidation by PPARα activation favors the formation of unconjugated bile acids

• Published in: Journal of lipid research Vol. 45; no. 6; pp. 1051 - 1060
• Main Authors: Solaas, Karianne, Kase, B. Frode, Pham, Viet, Bamberg, Krister, Hunt, Mary C., Alexson, Stefan E.H.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2004; Elsevier
• Subjects: Acyltransferases - genetics; Acyltransferases - metabolism; Amides - metabolism; Animals; bile acid-coenzyme A:amino acid N-acyltransferase; bile acid-coenzyme thioesterase; Bile Acids and Salts - chemistry; Bile Acids and Salts - metabolism; Catalase - metabolism; cholestyramine; Cytosol - metabolism; DNA-Binding Proteins - antagonists & inhibitors; DNA-Binding Proteins - metabolism; Down-Regulation; farnesoid X receptor; Liver - drug effects; Liver - metabolism; Male; Medicin och hälsovetenskap; Mice; Mice, Knockout; peroxisome proliferator-activated receptor α; Peroxisomes - drug effects; Peroxisomes - enzymology; Peroxisomes - genetics; Peroxisomes - metabolism; PPAR alpha - deficiency; PPAR alpha - genetics; PPAR alpha - metabolism; Pyrimidines - pharmacology; Receptors, Cytoplasmic and Nuclear; RNA, Messenger - genetics; Subcellular Fractions - metabolism; Thiolester Hydrolases - metabolism; Transcription Factors - antagonists & inhibitors; Transcription Factors - metabolism; cholestyramine; bile acid-coenzyme A:amino acid N-acyltransferase; bile acid-coenzyme thioesterase; farnesoid X receptor; peroxisome proliferator-activated receptor α

In human liver, unconjugated bile acids can be formed by the action of bile acid-CoA thioesterases (BACTEs), whereas bile acid conjugation with taurine or glycine (amidation) is catalyzed by bile acid-CoA:amino acid N-acyltransferases (BACATs). Both pathways exist in peroxisomes and cytosol. Bile ac...