pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan
•Six chitosans with molecular weights ranging from 3.3 to 300kDa were prepared.•Combined effects of chitosan MW, temperature, pH on bacterial growth were studied.•The pH effects on water solubility and zeta potential of chitosans were examined.•Positive correlation between chitosan ZP and antibacter...
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Published in | Carbohydrate polymers Vol. 134; pp. 74 - 81 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
England
Elsevier Ltd
10.12.2015
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Subjects | |
Online Access | Get full text |
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Abstract | •Six chitosans with molecular weights ranging from 3.3 to 300kDa were prepared.•Combined effects of chitosan MW, temperature, pH on bacterial growth were studied.•The pH effects on water solubility and zeta potential of chitosans were examined.•Positive correlation between chitosan ZP and antibacterial activity was obtained.
Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3kDa were prepared by cellulase degradation of chitosan (300kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2kDa, which may explain the loss of their antibacterial activity at pH 7.0. |
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AbstractList | Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3 kDa were prepared by cellulase degradation of chitosan (300 kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2 kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2 kDa, which may explain the loss of their antibacterial activity at pH 7.0. Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3 kDa were prepared by cellulase degradation of chitosan (300 kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2 kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2 kDa, which may explain the loss of their antibacterial activity at pH 7.0.Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3 kDa were prepared by cellulase degradation of chitosan (300 kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2 kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2 kDa, which may explain the loss of their antibacterial activity at pH 7.0. •Six chitosans with molecular weights ranging from 3.3 to 300kDa were prepared.•Combined effects of chitosan MW, temperature, pH on bacterial growth were studied.•The pH effects on water solubility and zeta potential of chitosans were examined.•Positive correlation between chitosan ZP and antibacterial activity was obtained. Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3kDa were prepared by cellulase degradation of chitosan (300kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2kDa, which may explain the loss of their antibacterial activity at pH 7.0. Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3kDa were prepared by cellulase degradation of chitosan (300kDa) and ultrafiltration techniques. We examined the correlation between activity against Escherichia coli and Staphylococcus aureus and chitosan MW, and provided the underlying explanation. In acidic pH conditions, the chitosan activity increased with increasing MW, irrespective of the temperature and bacteria tested. However, at neutral pH, chitosan activity increased as the MW decreased, and little activity was observed for chitosans with MW >29.2kDa. At pH 5.0 and 6.0, chitosans exhibited good water solubility and zeta potential (ZP) decreased with the MW, whereas the solubility and ZP of the chitosans decreased with increasing MW at pH 7.0. Particularly, low solubility and negative ZP values were determined for chitosans with MW >29.2kDa, which may explain the loss of their antibacterial activity at pH 7.0. |
Author | Tsai, Guo Jane Wu, Guan-James Chang, Shun-Hsien Lin, Hong-Ting Victor |
Author_xml | – sequence: 1 givenname: Shun-Hsien orcidid: 0000-0002-4044-9860 surname: Chang fullname: Chang, Shun-Hsien organization: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, ROC – sequence: 2 givenname: Hong-Ting Victor surname: Lin fullname: Lin, Hong-Ting Victor organization: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, ROC – sequence: 3 givenname: Guan-James surname: Wu fullname: Wu, Guan-James organization: Department of Food Science, National Penghu University of Science and Technology, Penghu, Taiwan, ROC – sequence: 4 givenname: Guo Jane orcidid: 0000-0002-5605-5495 surname: Tsai fullname: Tsai, Guo Jane email: b0090@mail.ntou.edu.tw organization: Department of Food Science, National Taiwan Ocean University, Keelung, Taiwan, ROC |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26428102$$D View this record in MEDLINE/PubMed |
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Keywords | Antibacterial activity Chitosan Zeta potential Molecular weight Solubility |
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Snippet | •Six chitosans with molecular weights ranging from 3.3 to 300kDa were prepared.•Combined effects of chitosan MW, temperature, pH on bacterial growth were... Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3 kDa were prepared by cellulase degradation of chitosan (300 kDa) and... Six chitosans with molecular weights (MWs) of 300, 156, 72.1, 29.2, 7.1, and 3.3kDa were prepared by cellulase degradation of chitosan (300kDa) and... |
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SubjectTerms | Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial activity antibacterial properties bacteria Chitosan Chitosan - analogs & derivatives Chitosan - pharmacology endo-1,4-beta-glucanase Escherichia coli Escherichia coli - drug effects Escherichia coli Infections - prevention & control Food Microbiology - methods Humans Hydrogen-Ion Concentration Microbial Sensitivity Tests Molecular Weight Solubility Staphylococcal Infections - prevention & control Staphylococcus aureus Staphylococcus aureus - drug effects Static Electricity temperature ultrafiltration water solubility Zeta potential |
Title | pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan |
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