Comparative hydrolysis and fermentation of sugarcane and agave bagasse

• Published in: Bioresource technology Vol. 100; no. 3; pp. 1238 - 1245
• Main Authors: Hernández-Salas, J.M., Villa-Ramírez, M.S., Veloz-Rendón, J.S., Rivera-Hernández, K.N., González-César, R.A., Plascencia-Espinosa, M.A., Trejo-Estrada, S.R.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2009; [New York, NY]: Elsevier Ltd; Elsevier
• Subjects: Agave; Agave - chemistry; Agave - metabolism; Agave atrovirens; alcoholic fermentation; Bagasse; beta-glucanase; biofuels; Biological and medical sciences; Biotechnology; Cellulose - chemistry; Cellulose - metabolism; delignification; enzymatic hydrolysis; Ethanol - chemistry; Ethanol - metabolism; ethanol production; Fermentation; Food industries; Fundamental and applied biological sciences. Psychology; glucose; hydrochloric acid; Hydrolysis; lignocellulose; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; renewable energy sources; Saccharomyces cerevisiae; Saccharomyces cerevisiae - metabolism; Saccharum - chemistry; Saccharum - metabolism; Saccharum officinarum; Sugarcane; sugarcane bagasse; sugars; Use and upgrading of agricultural and food by-products. Biotechnology; Hydrolysis; Fermentation; Sugarcane; Bagasse; Agave; Saccharum; Monocotyledones; Sugar plant; Succulent plant; Gramineae; Angiospermae; Agavaceae; Spermatophyta; Sugar cane
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2006.09.062

Sugarcane and agave bagasse samples were hydrolyzed with either mineral acids (HCl), commercial glucanases or a combined treatment consisting of alkaline delignification followed by enzymatic hydrolysis. Acid hydrolysis of sugar cane bagasse yielded a higher level of reducing sugars (37.21% for depithed bagasse and 35.37% for pith bagasse), when compared to metzal or metzontete (agave pinecone and leaves, 5.02% and 9.91%, respectively). An optimized enzyme formulation was used to process sugar cane bagasse, which contained Celluclast, Novozyme and Viscozyme L. From alkaline–enzymatic hydrolysis of sugarcane bagasse samples, a reduced level of reducing sugar yield was obtained (11–20%) compared to agave bagasse (12–58%). Selected hydrolyzates were fermented with a non-recombinant strain of Saccharomyces cerevisiae. Maximum alcohol yield by fermentation (32.6%) was obtained from the hydrolyzate of sugarcane depithed bagasse. Hydrolyzed agave waste residues provide an increased glucose decreased xylose product useful for biotechnological conversion.