Investigation of optimal procedures for storage and use of plasma samples suitable for gene doping tests

• Published in: Journal of Equine Science Vol. 34; no. 2; pp. 21 - 27
• Main Authors: ISHIGE, Taichiro, NAGATA, Shun-ichi, TAKAHASHI, Yuji, OHNUMA, Aoi, HIROTA, Kei-ichi, TOZAKI, Teruaki, KIKUCHI, Mio, KAKOI, Hironaga
• Format: Journal Article
• Language: English
• Published: Japan Japanese Society of Equine Science 01.06.2023; Japan Science and Technology Agency; The Japanese Society of Equine Science
• Subjects: Blood; Blood cells; Centrifugation; Cryopreservation; Doping; gene doping; Genes; Horse sports; Polymerase chain reaction; Refrigeration; Room temperature; sample splitting; Storage; Thawing; Thoroughbred; transgene; Transgenes; gene doping; transgene; sample splitting; Thoroughbred

Gene doping, which is prohibited in horseracing and equestrian sports, can be performed by introducing exogenous genes, known as transgenes, into the bodies of postnatal animals. To detect exogenous genes, a method utilizing quantitative polymerase chain reaction (qPCR) with a hydrolysis probe was d...