genomic binding sites of a noncoding RNA

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 51; pp. 20497 - 20502
• Main Authors: Simon, Matthew D, Wang, Charlotte I, Kharchenko, Peter V, West, Jason A, Chapman, Brad A, Alekseyenko, Artyom A, Borowsky, Mark L, Kuroda, Mitzi I, Kingston, Robert E
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 20.12.2011; National Acad Sciences
• Subjects: Amino Acid Motifs; Animals; Binding Sites; Biological Sciences; Charts; Chromatin; Chromatin - chemistry; Chromatin - genetics; Chromatin Immunoprecipitation; Compensation; crosslinking; DNA; Dosage compensation; Dosage Compensation, Genetic; Drosophila; Drosophila - genetics; Drosophila Proteins - genetics; Gene mapping; Genes; Genetic loci; Genomes; Genomics; humans; Hybridization; Hybridization analysis; Immunoprecipitation; Insects; Male; Models, Genetic; non-coding RNA; Nucleic Acid Hybridization; Peptide mapping; precipitin tests; Proteins; Ribonuclease H - chemistry; Ribonucleic acid; RNA; RNA, Untranslated - genetics; RNA-Binding Proteins - genetics

Long noncoding RNAs (lncRNAs) have important regulatory roles and can function at the level of chromatin. To determine where lncRNAs bind to chromatin, we developed capture hybridization analysis of RNA targets (CHART), a hybridization-based technique that specifically enriches endogenous RNAs along...