Multistep Fractionation and Mass Spectrometry Reveal Zwitterionic and Anionic Modifications of the N- and O-glycans of a Marine Snail

• Published in: Molecular & cellular proteomics Vol. 15; no. 2; pp. 573 - 597
• Main Authors: Eckmair, Barbara, Jin, Chunsheng, Abed-Navandi, Daniel, Paschinger, Katharina
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2016; The American Society for Biochemistry and Molecular Biology
• Subjects: 2-dimensional sugar map; acetylglucosamine residue; Amino Acid Transport Systems, Neutral - genetics; Animals; Anions; Biochemistry & Molecular Biology; biomphalaria-glabrata; caenorhabditis-elegans; Cell and Molecular Biology; Cell- och molekylärbiologi; Chromatography, High Pressure Liquid; crassostrea-virginica; dictyostelium-discoideum; Fucose - metabolism; Glycomics; linked carbohydrate chains; lymnaea-stagnalis; Mannose - metabolism; Mass Spectrometry; oyster; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - genetics; Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase - metabolism; performance liquid-chromatography; Polysaccharides - biosynthesis; Polysaccharides - genetics; Regular; schistosoma-mansoni; Snails - genetics; Snails - metabolism

Various studies in the past have revealed that molluscs can produce a wide range of rather complex N-glycan structures, which vary from those occurring in other invertebrate animals; particularly methylated glycans have been found in gastropods, and there are some reports of anionic glycans in bival...