Methods for fractionation, separation and profiling of proteins and peptides

• Published in: Electrophoresis Vol. 23; no. 17; pp. 3048 - 3061
• Main Authors: Issaq, Haleem J., Conrads, Thomas P., Janini, George M., Veenstra, Timothy D.
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.09.2002; WILEY‐VCH Verlag
• Subjects: Animals; Chromatography - instrumentation; Chromatography - methods; Electrophoresis - instrumentation; Electrophoresis - methods; Equipment Design; Fractionation; Humans; Multidimensional separations; Peptides; Peptides - analysis; Peptides - isolation & purification; Proteins; Proteins - analysis; Proteins - isolation & purification; Proteomics; Review

In the last few years there has been an increased effort to develop technologies capable of identifying and quantifying large numbers of proteins expressed within a cell system (i.e., the proteome). The complexity of the mixtures being analyzed has made the development of effective fractionation and...