Crystallization and preliminary X-ray analysis of l-azetidine-2-carboxylate hydrolase from Pseudomonas sp. strain A2C

• Published in: Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 66; no. 7; pp. 801 - 804
• Main Authors: Toyoda, Mayuko, Jitsumori, Keiji, Mikami, Bunzo, Wackett, Lawrence P., Kurihara, Tatsuo, Esaki, Nobuyoshi
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.06.2010
• Subjects: Amino Acid Sequence; Crystallization; Crystallization Communications; Crystallography, X-Ray; Hydrolases - chemistry; l-azetidine-2-carboxylate hydrolase; Molecular Sequence Data; Pseudomonas; Pseudomonas - enzymology; Pseudomonas sp. strain A2C; Sequence Alignment

l‐Azetidine‐2‐carboxylate hydrolase from Pseudomonas sp. strain A2C catalyzes a ring‐opening reaction that detoxifies l‐azetidine‐2‐carboxylate, an analogue of l‐proline. Recombinant l‐azetidine‐2‐carboxylate hydrolase was overexpressed, purified and crystallized using polyethylene glycol and magnes...