An Efficient One-Pot Four-Segment Condensation Method for Protein Chemical Synthesis

• Published in: Angewandte Chemie International Edition Vol. 54; no. 19; pp. 5713 - 5717
• Main Authors: Tang, Shan, Si, Yan-Yan, Wang, Zhi-Peng, Mei, Kun-Rong, Chen, Xin, Cheng, Jing-Yuan, Zheng, Ji-Shen, Liu, Lei
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 04.05.2015; WILEY‐VCH Verlag; Wiley; Wiley Subscription Services, Inc
• Edition: International ed. in English
• Subjects: Activated; Adjustment; Chemical synthesis; Chemistry; Chemistry, Multidisciplinary; Chemokine CCL21 - chemical synthesis; Chemokine CCL21 - chemistry; Condensation; Condensing; Cysteine; Human; Humans; native chemical ligation; Peptides; Peptides - chemistry; Physical Sciences; Plant Proteins - chemical synthesis; Plant Proteins - chemistry; protecting groups; protein chemical synthesis; Proteins; Science & Technology; Segments; solid-phase synthesis; Synthesis; SITE; HYDRAZIDES; CCL21; PEPTIDE LIGATION-DESULFURIZATION; X-RAY-STRUCTURE; peptides; protein chemical synthesis; CRAMBIN; solid-phase synthesis; N-SULFANYLETHYLANILIDE PEPTIDE; STRATEGIES; protecting groups; CHEMOKINE; native chemical ligation

Successive peptide ligation using a one‐pot method can improve the efficiency of protein chemical synthesis. Although one‐pot three‐segment ligation has enjoyed widespread application, a robust method for one‐pot four‐segment ligation had to date remained undeveloped. Herein we report a new one‐pot...