Prolonged ex vivo culture of human bone marrow mesenchymal stem cells influences their supportive activity toward NOD/SCID-repopulating cells and committed progenitor cells of B lymphoid and myeloid lineages

• Published in: Haematologica (Roma) Vol. 95; no. 1; pp. 47 - 56
• Main Authors: BRIQUET, Alexandra, DUBOIS, Sophie, BEKAERT, Sandrine, DOLHET, Marie, BEGUIN, Yves, GOTHOT, André
• Format: Journal Article Web Resource
• Language: English
• Published: Pavia Ferrata Storti Foundation 01.01.2010
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Biological and medical sciences; Bone Marrow Cells - cytology; Bone Marrow Cells - physiology; Bone marrow, stem cells transplantation. Graft versus host reaction; Cell Culture Techniques - methods; Cell Differentiation - physiology; Cell Lineage - physiology; Cell Proliferation; Hematologic and hematopoietic diseases; Hematology; human bone marrow mesenchymal stem cells; Human health sciences; Humans; Hématologie; Lymphoid Progenitor Cells - cytology; Lymphoid Progenitor Cells - physiology; Medical sciences; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - physiology; Mice; Mice, Inbred NOD; Mice, SCID; Myeloid Progenitor Cells - cytology; Myeloid Progenitor Cells - physiology; NOD/SCID-repopulating cells; Original; progenitor cells of B lymphoid; Sciences de la santé humaine; Time Factors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy; Human; Cell proliferation; Cell culture; Lymphoid cell; Hematology; Stem cell; Hematopoietic cell; NOD/SCID mouse; B-Lymphocyte; Mesenchymal cell; Myeloid cell; progenitor cells of B lymphoid; NOD/SCID-repopulating cells; Ex vivo; Bone marrow; human bone marrow mesenchymal stem cells; Prolonged

Bone marrow mesenchymal stem cells support proliferation and differentiation of hematopoietic progenitor cells in vitro. Since these cells constitute a rare subset of bone marrow cells, mesenchymal stem cell preparations for clinical purposes require a preparative step of ex vivo multiplication. The...