Evaluation of L-Alanine Metabolism in Bacteria and Whole-Body Distribution with Bacterial Infection Model Mice
The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators...
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Published in | International journal of molecular sciences Vol. 24; no. 5; p. 4775 |
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Main Authors | , , , , , , , , , , , , , |
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Abstract | The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators of bacterial growth activity by clarifying which amino acids are taken up by bacteria during the various growth phases. In addition, we examined the amino acid transport mechanisms that are employed by bacteria based on the accumulation of labeled amino acids, Na
dependence, and inhibitory effects using a specific inhibitor of system A. We found that
H-L-Ala accurately reflects the proliferative activity of
K-12 and pathogenic EC-14 in vitro. This accumulation in
could be attributed to the amino acid transport systems being different from those found in human tumor cells. Moreover, biological distribution assessed in infection model mice with EC-14 using
H-L-Ala showed that the ratio of
H-L-Ala accumulated in infected muscle to that in control muscle was 1.20. By detecting the growth activity of bacteria in the body that occurs during the early stages of infection by nuclear imaging, such detection methods may result in expeditious diagnostic treatments for infectious diseases. |
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AbstractList | The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators of bacterial growth activity by clarifying which amino acids are taken up by bacteria during the various growth phases. In addition, we examined the amino acid transport mechanisms that are employed by bacteria based on the accumulation of labeled amino acids, Na+ dependence, and inhibitory effects using a specific inhibitor of system A. We found that 3H-L-Ala accurately reflects the proliferative activity of Escherichia coli K-12 and pathogenic EC-14 in vitro. This accumulation in E. coli could be attributed to the amino acid transport systems being different from those found in human tumor cells. Moreover, biological distribution assessed in infection model mice with EC-14 using 3H-L-Ala showed that the ratio of 3H-L-Ala accumulated in infected muscle to that in control muscle was 1.20. By detecting the growth activity of bacteria in the body that occurs during the early stages of infection by nuclear imaging, such detection methods may result in expeditious diagnostic treatments for infectious diseases. The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators of bacterial growth activity by clarifying which amino acids are taken up by bacteria during the various growth phases. In addition, we examined the amino acid transport mechanisms that are employed by bacteria based on the accumulation of labeled amino acids, Na dependence, and inhibitory effects using a specific inhibitor of system A. We found that H-L-Ala accurately reflects the proliferative activity of K-12 and pathogenic EC-14 in vitro. This accumulation in could be attributed to the amino acid transport systems being different from those found in human tumor cells. Moreover, biological distribution assessed in infection model mice with EC-14 using H-L-Ala showed that the ratio of H-L-Ala accumulated in infected muscle to that in control muscle was 1.20. By detecting the growth activity of bacteria in the body that occurs during the early stages of infection by nuclear imaging, such detection methods may result in expeditious diagnostic treatments for infectious diseases. The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators of bacterial growth activity by clarifying which amino acids are taken up by bacteria during the various growth phases. In addition, we examined the amino acid transport mechanisms that are employed by bacteria based on the accumulation of labeled amino acids, Na[sup.+] dependence, and inhibitory effects using a specific inhibitor of system A. We found that [sup.3]H-L-Ala accurately reflects the proliferative activity of Escherichia coli K-12 and pathogenic EC-14 in vitro. This accumulation in E. coli could be attributed to the amino acid transport systems being different from those found in human tumor cells. Moreover, biological distribution assessed in infection model mice with EC-14 using [sup.3]H-L-Ala showed that the ratio of [sup.3]H-L-Ala accumulated in infected muscle to that in control muscle was 1.20. By detecting the growth activity of bacteria in the body that occurs during the early stages of infection by nuclear imaging, such detection methods may result in expeditious diagnostic treatments for infectious diseases. The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To facilitate prompt and accurate diagnosis and treatment of infectious disease, we investigated the potential of amino acids for use as indicators of bacterial growth activity by clarifying which amino acids are taken up by bacteria during the various growth phases. In addition, we examined the amino acid transport mechanisms that are employed by bacteria based on the accumulation of labeled amino acids, Na + dependence, and inhibitory effects using a specific inhibitor of system A. We found that 3 H-L-Ala accurately reflects the proliferative activity of Escherichia coli K-12 and pathogenic EC-14 in vitro. This accumulation in E. coli could be attributed to the amino acid transport systems being different from those found in human tumor cells. Moreover, biological distribution assessed in infection model mice with EC-14 using 3 H-L-Ala showed that the ratio of 3 H-L-Ala accumulated in infected muscle to that in control muscle was 1.20. By detecting the growth activity of bacteria in the body that occurs during the early stages of infection by nuclear imaging, such detection methods may result in expeditious diagnostic treatments for infectious diseases. |
Audience | Academic |
Author | Mizutani, Asuka Shikano, Naoto Kobayashi, Masato Sato, Kakeru Ohata, Shusei Kawai, Keiichi Matsue, Miki Nishii, Ryuichi Nishi, Kodai Muranaka, Yuka Kondo, Ami Nishiyama, Yuri Okamoto, Shigefumi Yamazaki, Kana |
AuthorAffiliation | 5 Department of Radioisotope Medicine, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan 8 Advanced Health Care Science Research Unit, Innovative Integrated Bio-Research Core Institute for Frontier Science Initiative, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan 4 Department of Radiation Technology, Toyama Red Cross Hospital, 2-1-58 Ushijimahonmachi, Toyama 930-8562, Japan 6 Department of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan 1 Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan 2 Ishikawa Prefectural Institute of Public Health and Environmental Science, 1-11 Taiyogaoka, Kanazawa 920-1154, Japan 7 Department of Radiological Sciences, Ibaraki Prefectural University of Heal |
AuthorAffiliation_xml | – name: 1 Division of Health Sciences, Graduate School of Medical Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan – name: 2 Ishikawa Prefectural Institute of Public Health and Environmental Science, 1-11 Taiyogaoka, Kanazawa 920-1154, Japan – name: 6 Department of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan – name: 9 Biomedical Imaging Research Center, University of Fukui, 23-3 Matsuokashimoaizuki, Eiheiji, Fukui 910-1193, Japan – name: 8 Advanced Health Care Science Research Unit, Innovative Integrated Bio-Research Core Institute for Frontier Science Initiative, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan – name: 7 Department of Radiological Sciences, Ibaraki Prefectural University of Health Sciences, 4669-2 Ami, Inashiki 300-0394, Japan – name: 3 Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa University, 5-11-80 Kodatsuno, Kanazawa 920-0942, Japan – name: 5 Department of Radioisotope Medicine, Atomic Bomb Disease Institute, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan – name: 4 Department of Radiation Technology, Toyama Red Cross Hospital, 2-1-58 Ushijimahonmachi, Toyama 930-8562, Japan |
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Keywords | bacterial imaging alanine nuclear medicine imaging bacterial infection amino acids |
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Snippet | The World Health Organization has cautioned that antimicrobial resistance (AMR) will be responsible for an estimated 10 million deaths annually by 2050. To... |
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SubjectTerms | Accumulation Alanine Alanine - metabolism Amino acids Amino Acids - metabolism Animal models Animals Antimicrobial resistance Bacteria bacterial imaging bacterial infection Bacterial Infections Cancer E coli Escherichia coli Escherichia coli - metabolism Escherichia coli K12 - metabolism Health aspects Humans Infections Infectious diseases L-Alanine Mice Muscles nuclear medicine imaging Pathogens Physiological aspects Transportation systems Tumor cells |
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Title | Evaluation of L-Alanine Metabolism in Bacteria and Whole-Body Distribution with Bacterial Infection Model Mice |
URI | https://www.ncbi.nlm.nih.gov/pubmed/36902204 https://www.proquest.com/docview/2785219166/abstract/ https://search.proquest.com/docview/2786094717 https://pubmed.ncbi.nlm.nih.gov/PMC10002749 https://doaj.org/article/f165cbf0b86e4715b5cdbdd5cedf4f04 |
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