Rheological analysis of sputum from patients with chronic bronchial diseases
Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum sampl...
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| Published in | Scientific reports Vol. 10; no. 1; pp. 15685 - 10 |
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| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
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Nature Publishing Group UK
24.09.2020
Nature Publishing Group Nature Portfolio |
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| ISSN | 2045-2322 2045-2322 |
| DOI | 10.1038/s41598-020-72672-6 |
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| Abstract | Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases. |
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| AbstractList | Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases. Abstract Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases. Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases.Bronchial diseases are characterised by the weak efficiency of mucus transport through the lower airways, leading in some cases to the muco-obstruction of bronchi. It has been hypothesised that this loss of clearance results from alterations in the mucus rheology, which are reflected in sputum samples collected from patients, making sputum rheology a possible biophysical marker of these diseases and their evolution. However, previous rheological studies have focused on quasi-static viscoelastic (linear storage and loss moduli) properties only, which are not representative of the mucus mobilisation within the respiratory tract. In this paper, we extend this approach further, by analysing both quasi-static and some dynamic (flow point) properties, to assess their usability and relative performance in characterising several chronic bronchial diseases (asthma, chronic obstructive pulmonary disease, and cystic fibrosis) and distinguishing them from healthy subjects. We demonstrate that pathologies influence substantially the linear and flow properties. Linear moduli are weakly condition-specific and even though the corresponding ranges overlap, distinct levels can be identified. This directly relates to the specific mucus structure in each case. In contrast, the flow point is found to strongly increase in muco-obstructive diseases, which may reflect the complete failure of mucociliary clearance causing episodic obstructions. These results suggest that the analysis of quasi-static and dynamic regimes in sputum rheology is in fact useful as these regimes provide complementary markers of chronic bronchial diseases. |
| ArticleNumber | 15685 |
| Author | Obamba, Martinien Quétant, Sébastien Robert de Saint Vincent, Matthieu Ghiringhelli, Étienne Bochu, Philippe Cracowski, Claire Camara, Boubou Patarin, Jérémy Darsy, Guillaume Cracowski, Jean-Luc |
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| Cites_doi | 10.3233/BIR-1989-26406 10.1016/j.addr.2008.09.012 10.1378/chest.114.1.171 10.1183/09031936.02.00000302 10.1203/00006450-198102000-00007 10.1183/09031936.96.09040769 10.1017/jfm.2017.352 10.3233/BIR-1987-24606 10.1136/thx.51.2.119 10.3389/fphy.2019.00019 10.1073/pnas.87.23.9188 10.1056/NEJMra1813799 10.1016/j.chest.2018.03.005 10.1172/jci.insight.88198 10.1152/jappl.1977.42.6.797 10.1103/PhysRevE.83.011921 10.1111/j.1365-2362.1985.tb00290.x 10.1529/biophysj.106.094698 10.1164/arrd.1975.112.3.341 10.3233/BIR-17141 10.1186/1465-9921-10-63 10.1007/BF01976227 10.1164/arrd.1968.98.3.380 10.1371/journal.pone.0082297 10.1152/jappl.1985.58.6.1776 10.1186/s12890-018-0661-1 10.1172/JCI73469 10.1038/s41598-018-20882-4 10.1073/pnas.1811787115 10.1016/j.resp.2018.04.006 10.1164/ajrccm.156.1.9512074 10.1183/09031936.95.08030381 |
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| References | AppEMSputum rheology changes in cystic fibrosis lung disease following two different types of physiotherapyChest19981141711771:STN:280:DyaK1czjslahsQ%3D%3D10.1378/chest.114.1.1719674466 RadtkeTThe many ways sputum flows—dealing with high within-subject variability in cystic fibrosis sputum rheologyRespir. Physiol. Neurobiol.2018254363910.1016/j.resp.2018.04.00629684501 KingMMacklemPTRheological properties of microliter quantities of normal mucusJ. Appl. Physiol.1977427978021:STN:280:DyaE2s3islKjsA%3D%3D10.1152/jappl.1977.42.6.797142075 NettleCLinear rheology as a potential monitoring tool for sputum in patients with chronic obstructive pulmonary disease (COPD)Biorheology20185467801:STN:280:DC%2BC1Mzlt1Gnsg%3D%3D10.3233/BIR-1714129278868 ButtonBRoles of mucus adhesion and cohesion in cough clearanceProc. Natl. Acad. Sci. USA201811512501125061:CAS:528:DC%2BC1cXitl2hsrjF10.1073/pnas.181178711530420506 DentonRForsmanWHwangSHLittMMillerCEViscoelasticity of mucusAm. Rev. Respir. Dis.1968983803911:STN:280:DyaF1czpvVKhuw%3D%3D10.1164/arrd.1968.98.3.3805673097 KingMZahmJ-MPierrotDVaquez-GirodSPuchelleEThe role of mucus gel viscosity, spinnability, and adhesive properties in clearance by simulated coughBiorheology1989267377451:STN:280:DyaK3c7itlWjug%3D%3D10.3233/BIR-1989-26406 KingMBrockGLundellCClearance of mucus by simulated coughJ. Appl. Physiol.198558177617821:STN:280:DyaL2M3jsVaktQ%3D%3D10.1152/jappl.1985.58.6.17764008399 BoucherRCMuco-obstructive lung diseasesN. Engl. J. Med.2019380194119531:CAS:528:DC%2BC1MXhtVWisL7O10.1056/NEJMra181379931091375 KingMDasguptaBTomkiewiczRPBrownNERheology of cystic fibrosis sputum after in vitro treatment with hypertonic saline alone and in combination with recombinant human deoxyribonuclease IAm. J. Respir. Crit. Care Med.19971561731771:STN:280:DyaK2sznsVaqtg%3D%3D10.1164/ajrccm.156.1.95120749230743 TomaiuoloGA new method to improve the clinical evaluation of cystic fibrosis patients by mucus viscoelastic propertiesPLoS ONE20149e822972014PLoSO...982297T1:CAS:528:DC%2BC2cXivFOis7Y%3D10.1371/journal.pone.0082297244041293880261 KhelloufiM-KSpatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epitheliumSci. Rep.2018824472018NatSR...8.2447K1:CAS:528:DC%2BC1cXhs1eiu7rJ10.1038/s41598-018-20882-4294029605799192 Ghiringhelli, E. & Patarin, J. Method for processing a sputum sample and method for the rheometric measurement of a preprocessed sample of this kind. International Patent WO/2019/020932. https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2019020932 (2019). DulfanoMJAdlerKBPhysical properties of sputum VII. Rheologic properties and mucociliary transportAm. Rev. Respir. Dis.19751123413471:STN:280:DyaE28%2FhslSqsg%3D%3D10.1164/arrd.1975.112.3.3411080651 DuncanGAMicrostructural alterations of sputum in cystic fibrosis lung diseaseJCI Insight20161e8819810.1172/jci.insight.88198278125405085601 MaJTTangCKangLVoynowJARubinBKCystic fibrosis sputum rheology correlates with both acute and longitudinal changes in lung functionChest201815437037710.1016/j.chest.2018.03.00529559310 JoryMMucus microrheology measured on human bronchial epithelium cultureFront. Phys.201971910.3389/fphy.2019.00019 Puchelle, E., Girard, F. & Zahm, J.-M. Rheology of bronchial secretions and mucociliary transport (author’s transl). Bull. Eur. Physiopathol. Respir.12, 771–779 (1976). PuchelleEZahmJMQuemadaDRheological properties controlling mucociliary frequency and respiratory mucus transportBiorheology1987245575631:STN:280:DyaL1M%2FgtFSrug%3D%3D10.3233/BIR-1987-24606 ShahPIn vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosisThorax1996511191251:STN:280:DyaK283ks1Khsg%3D%3D10.1136/thx.51.2.119 Grenoble University Hospital. Assessment of Rheological Parameters of Human Sputum (RHEOMUCO). https://clinicaltrials.gov/show/NCT02682290 (2016). World Health Organization. The top 10 causes of death. https://www.who.int/en/news-room/fact-sheets/detail/the-top-10-causes-of-death (2018). PuchelleEEffects of rhDNase on purulent airway secretions in chronic bronchitisEur. Respir. J.199697657691:CAS:528:DyaK28Xjslens7w%3D10.1183/09031936.96.090407698726943 Chateau, S., Favier, J., D’Ortona, U. & Poncet, S. Transport efficiency of metachronal waves in 3D cilium arrays immersed in a two-phase flow. J. Fluid Mech.824, 931–961. https://doi.org/10.1017/jfm.2017.352 (2017). HwangSHLittMForsmanWCRheological properties of mucusRheol. Acta1969843844810.1007/BF01976227 ZahmJ-MDose-dependent in vitro effect of recombinant human DNase on rheological and transport properties of cystic fibrosis respiratory mucusEur. Respir. J.199583813861:STN:280:DyaK2MzgvFeltQ%3D%3D10.1183/09031936.95.080303817789481 KingMIs cystic fibrosis mucus abnormal?Pediatr. Res.1981151201221:STN:280:DyaL3M3ks1CqtA%3D%3D10.1203/00006450-198102000-000077254936 PuchelleEJacquotJBeckGZahmJMGalabertCRheological and transport properties of airway secretions in cystic fibrosis—relationships with the degree of infection and severity of the diseaseEur. J. Clin. Invest.1985153893941:STN:280:DyaL287msVGhtQ%3D%3D10.1111/j.1365-2362.1985.tb00290.x HendersonAGCystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressureJ. Clin. Invest.2014124304730601:CAS:528:DC%2BC2cXhtFOht77F10.1172/JCI73469248928084072023 RadtkeTAcute effects of combined exercise and oscillatory positive expiratory pressure therapy on sputum properties and lung diffusing capacity in cystic fibrosis: a randomized, controlled, crossover trialBMC Pulm. Med.201818991:CAS:528:DC%2BC1MXkvFait70%3D10.1186/s12890-018-0661-1298987046000950 NortonMMRobinsonRJWeinsteinSJModel of ciliary clearance and the role of mucus rheologyPhys. Rev. E2011830119212011PhRvE..83a1921N1:CAS:528:DC%2BC3MXhsFSgtbo%3D10.1103/PhysRevE.83.011921 ChanezPSputum inductionEur. Respir. J.2002203s8s10.1183/09031936.02.00000302 TeffZPrielZGheberLAForces applied by cilia measured on explants from mucociliary tissueBiophys. J.200792181318232007BpJ....92.1813T1:CAS:528:DC%2BD2sXit1alsrc%3D10.1529/biophysj.106.09469817142280 SerisierDJCarrollMPShuteJKYoungSAMacrorheology of cystic fibrosis, chronic obstructive pulmonary disease & normal sputumRespir. Res.2009106310.1186/1465-9921-10-63195806502712464 LaiSKWangY-YWirtzDHanesJMicro- and macrorheology of mucusAdv. Drug Deliv. Rev.200961861001:CAS:528:DC%2BD1MXit1Ohtrg%3D10.1016/j.addr.2008.09.012191668892736374 ShakSCaponDJHellmissRMarstersSABakerCLRecombinant human DNase I reduces the viscosity of cystic fibrosis sputumProc. Natl. Acad. Sci. USA199087918891921990PNAS...87.9188S1:CAS:528:DyaK3MXlsFKjtQ%3D%3D10.1073/pnas.87.23.91882251263 J-M Zahm (72672_CR14) 1995; 8 RC Boucher (72672_CR3) 2019; 380 E Puchelle (72672_CR10) 1987; 24 T Radtke (72672_CR19) 2018; 18 MM Norton (72672_CR33) 2011; 83 E Puchelle (72672_CR9) 1985; 15 M King (72672_CR12) 1989; 26 SK Lai (72672_CR2) 2009; 61 JT Ma (72672_CR24) 2018; 154 GA Duncan (72672_CR22) 2016; 1 P Shah (72672_CR15) 1996; 51 M-K Khelloufi (72672_CR31) 2018; 8 P Chanez (72672_CR28) 2002; 20 C Nettle (72672_CR29) 2018; 54 72672_CR36 72672_CR35 B Button (72672_CR27) 2018; 115 72672_CR34 M King (72672_CR11) 1981; 15 M King (72672_CR17) 1997; 156 S Shak (72672_CR13) 1990; 87 EM App (72672_CR18) 1998; 114 DJ Serisier (72672_CR20) 2009; 10 M Jory (72672_CR30) 2019; 7 M King (72672_CR7) 1977; 42 MJ Dulfano (72672_CR8) 1975; 112 T Radtke (72672_CR25) 2018; 254 R Denton (72672_CR4) 1968; 98 SH Hwang (72672_CR5) 1969; 8 72672_CR6 AG Henderson (72672_CR21) 2014; 124 G Tomaiuolo (72672_CR23) 2014; 9 M King (72672_CR26) 1985; 58 Z Teff (72672_CR32) 2007; 92 E Puchelle (72672_CR16) 1996; 9 72672_CR1 |
| References_xml | – reference: PuchelleEEffects of rhDNase on purulent airway secretions in chronic bronchitisEur. Respir. J.199697657691:CAS:528:DyaK28Xjslens7w%3D10.1183/09031936.96.090407698726943 – reference: PuchelleEZahmJMQuemadaDRheological properties controlling mucociliary frequency and respiratory mucus transportBiorheology1987245575631:STN:280:DyaL1M%2FgtFSrug%3D%3D10.3233/BIR-1987-24606 – reference: AppEMSputum rheology changes in cystic fibrosis lung disease following two different types of physiotherapyChest19981141711771:STN:280:DyaK1czjslahsQ%3D%3D10.1378/chest.114.1.1719674466 – reference: RadtkeTThe many ways sputum flows—dealing with high within-subject variability in cystic fibrosis sputum rheologyRespir. Physiol. Neurobiol.2018254363910.1016/j.resp.2018.04.00629684501 – reference: HwangSHLittMForsmanWCRheological properties of mucusRheol. Acta1969843844810.1007/BF01976227 – reference: KingMZahmJ-MPierrotDVaquez-GirodSPuchelleEThe role of mucus gel viscosity, spinnability, and adhesive properties in clearance by simulated coughBiorheology1989267377451:STN:280:DyaK3c7itlWjug%3D%3D10.3233/BIR-1989-26406 – reference: ChanezPSputum inductionEur. Respir. J.2002203s8s10.1183/09031936.02.00000302 – reference: Ghiringhelli, E. & Patarin, J. Method for processing a sputum sample and method for the rheometric measurement of a preprocessed sample of this kind. International Patent WO/2019/020932. https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2019020932 (2019). – reference: JoryMMucus microrheology measured on human bronchial epithelium cultureFront. Phys.201971910.3389/fphy.2019.00019 – reference: Grenoble University Hospital. Assessment of Rheological Parameters of Human Sputum (RHEOMUCO). https://clinicaltrials.gov/show/NCT02682290 (2016). – reference: SerisierDJCarrollMPShuteJKYoungSAMacrorheology of cystic fibrosis, chronic obstructive pulmonary disease & normal sputumRespir. Res.2009106310.1186/1465-9921-10-63195806502712464 – reference: TeffZPrielZGheberLAForces applied by cilia measured on explants from mucociliary tissueBiophys. J.200792181318232007BpJ....92.1813T1:CAS:528:DC%2BD2sXit1alsrc%3D10.1529/biophysj.106.09469817142280 – reference: NortonMMRobinsonRJWeinsteinSJModel of ciliary clearance and the role of mucus rheologyPhys. Rev. E2011830119212011PhRvE..83a1921N1:CAS:528:DC%2BC3MXhsFSgtbo%3D10.1103/PhysRevE.83.011921 – reference: KhelloufiM-KSpatiotemporal organization of cilia drives multiscale mucus swirls in model human bronchial epitheliumSci. Rep.2018824472018NatSR...8.2447K1:CAS:528:DC%2BC1cXhs1eiu7rJ10.1038/s41598-018-20882-4294029605799192 – reference: MaJTTangCKangLVoynowJARubinBKCystic fibrosis sputum rheology correlates with both acute and longitudinal changes in lung functionChest201815437037710.1016/j.chest.2018.03.00529559310 – reference: BoucherRCMuco-obstructive lung diseasesN. Engl. J. Med.2019380194119531:CAS:528:DC%2BC1MXhtVWisL7O10.1056/NEJMra181379931091375 – reference: Puchelle, E., Girard, F. & Zahm, J.-M. Rheology of bronchial secretions and mucociliary transport (author’s transl). Bull. Eur. Physiopathol. Respir.12, 771–779 (1976). – reference: KingMBrockGLundellCClearance of mucus by simulated coughJ. Appl. Physiol.198558177617821:STN:280:DyaL2M3jsVaktQ%3D%3D10.1152/jappl.1985.58.6.17764008399 – reference: KingMIs cystic fibrosis mucus abnormal?Pediatr. Res.1981151201221:STN:280:DyaL3M3ks1CqtA%3D%3D10.1203/00006450-198102000-000077254936 – reference: PuchelleEJacquotJBeckGZahmJMGalabertCRheological and transport properties of airway secretions in cystic fibrosis—relationships with the degree of infection and severity of the diseaseEur. J. Clin. Invest.1985153893941:STN:280:DyaL287msVGhtQ%3D%3D10.1111/j.1365-2362.1985.tb00290.x – reference: KingMMacklemPTRheological properties of microliter quantities of normal mucusJ. Appl. Physiol.1977427978021:STN:280:DyaE2s3islKjsA%3D%3D10.1152/jappl.1977.42.6.797142075 – reference: ButtonBRoles of mucus adhesion and cohesion in cough clearanceProc. Natl. Acad. Sci. USA201811512501125061:CAS:528:DC%2BC1cXitl2hsrjF10.1073/pnas.181178711530420506 – reference: World Health Organization. The top 10 causes of death. https://www.who.int/en/news-room/fact-sheets/detail/the-top-10-causes-of-death (2018). – reference: LaiSKWangY-YWirtzDHanesJMicro- and macrorheology of mucusAdv. Drug Deliv. Rev.200961861001:CAS:528:DC%2BD1MXit1Ohtrg%3D10.1016/j.addr.2008.09.012191668892736374 – reference: ShahPIn vivo effects of recombinant human DNase I on sputum in patients with cystic fibrosisThorax1996511191251:STN:280:DyaK283ks1Khsg%3D%3D10.1136/thx.51.2.119 – reference: HendersonAGCystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressureJ. Clin. Invest.2014124304730601:CAS:528:DC%2BC2cXhtFOht77F10.1172/JCI73469248928084072023 – reference: KingMDasguptaBTomkiewiczRPBrownNERheology of cystic fibrosis sputum after in vitro treatment with hypertonic saline alone and in combination with recombinant human deoxyribonuclease IAm. J. Respir. Crit. Care Med.19971561731771:STN:280:DyaK2sznsVaqtg%3D%3D10.1164/ajrccm.156.1.95120749230743 – reference: Chateau, S., Favier, J., D’Ortona, U. & Poncet, S. Transport efficiency of metachronal waves in 3D cilium arrays immersed in a two-phase flow. J. Fluid Mech.824, 931–961. https://doi.org/10.1017/jfm.2017.352 (2017). – reference: TomaiuoloGA new method to improve the clinical evaluation of cystic fibrosis patients by mucus viscoelastic propertiesPLoS ONE20149e822972014PLoSO...982297T1:CAS:528:DC%2BC2cXivFOis7Y%3D10.1371/journal.pone.0082297244041293880261 – reference: ZahmJ-MDose-dependent in vitro effect of recombinant human DNase on rheological and transport properties of cystic fibrosis respiratory mucusEur. Respir. J.199583813861:STN:280:DyaK2MzgvFeltQ%3D%3D10.1183/09031936.95.080303817789481 – reference: DulfanoMJAdlerKBPhysical properties of sputum VII. Rheologic properties and mucociliary transportAm. Rev. Respir. Dis.19751123413471:STN:280:DyaE28%2FhslSqsg%3D%3D10.1164/arrd.1975.112.3.3411080651 – reference: ShakSCaponDJHellmissRMarstersSABakerCLRecombinant human DNase I reduces the viscosity of cystic fibrosis sputumProc. Natl. Acad. Sci. USA199087918891921990PNAS...87.9188S1:CAS:528:DyaK3MXlsFKjtQ%3D%3D10.1073/pnas.87.23.91882251263 – reference: NettleCLinear rheology as a potential monitoring tool for sputum in patients with chronic obstructive pulmonary disease (COPD)Biorheology20185467801:STN:280:DC%2BC1Mzlt1Gnsg%3D%3D10.3233/BIR-1714129278868 – reference: DuncanGAMicrostructural alterations of sputum in cystic fibrosis lung diseaseJCI Insight20161e8819810.1172/jci.insight.88198278125405085601 – reference: DentonRForsmanWHwangSHLittMMillerCEViscoelasticity of mucusAm. Rev. Respir. Dis.1968983803911:STN:280:DyaF1czpvVKhuw%3D%3D10.1164/arrd.1968.98.3.3805673097 – reference: RadtkeTAcute effects of combined exercise and oscillatory positive expiratory pressure therapy on sputum properties and lung diffusing capacity in cystic fibrosis: a randomized, controlled, crossover trialBMC Pulm. Med.201818991:CAS:528:DC%2BC1MXkvFait70%3D10.1186/s12890-018-0661-1298987046000950 – volume: 26 start-page: 737 year: 1989 ident: 72672_CR12 publication-title: Biorheology doi: 10.3233/BIR-1989-26406 – volume: 61 start-page: 86 year: 2009 ident: 72672_CR2 publication-title: Adv. Drug Deliv. Rev. doi: 10.1016/j.addr.2008.09.012 – volume: 114 start-page: 171 year: 1998 ident: 72672_CR18 publication-title: Chest doi: 10.1378/chest.114.1.171 – volume: 20 start-page: 3s year: 2002 ident: 72672_CR28 publication-title: Eur. Respir. J. doi: 10.1183/09031936.02.00000302 – volume: 15 start-page: 120 year: 1981 ident: 72672_CR11 publication-title: Pediatr. Res. doi: 10.1203/00006450-198102000-00007 – volume: 9 start-page: 765 year: 1996 ident: 72672_CR16 publication-title: Eur. Respir. J. doi: 10.1183/09031936.96.09040769 – ident: 72672_CR34 doi: 10.1017/jfm.2017.352 – ident: 72672_CR36 – volume: 24 start-page: 557 year: 1987 ident: 72672_CR10 publication-title: Biorheology doi: 10.3233/BIR-1987-24606 – volume: 51 start-page: 119 year: 1996 ident: 72672_CR15 publication-title: Thorax doi: 10.1136/thx.51.2.119 – volume: 7 start-page: 19 year: 2019 ident: 72672_CR30 publication-title: Front. Phys. doi: 10.3389/fphy.2019.00019 – volume: 87 start-page: 9188 year: 1990 ident: 72672_CR13 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.87.23.9188 – volume: 380 start-page: 1941 year: 2019 ident: 72672_CR3 publication-title: N. Engl. J. Med. doi: 10.1056/NEJMra1813799 – volume: 154 start-page: 370 year: 2018 ident: 72672_CR24 publication-title: Chest doi: 10.1016/j.chest.2018.03.005 – volume: 1 start-page: e88198 year: 2016 ident: 72672_CR22 publication-title: JCI Insight doi: 10.1172/jci.insight.88198 – volume: 42 start-page: 797 year: 1977 ident: 72672_CR7 publication-title: J. Appl. Physiol. doi: 10.1152/jappl.1977.42.6.797 – volume: 83 start-page: 011921 year: 2011 ident: 72672_CR33 publication-title: Phys. Rev. E doi: 10.1103/PhysRevE.83.011921 – volume: 15 start-page: 389 year: 1985 ident: 72672_CR9 publication-title: Eur. J. Clin. Invest. doi: 10.1111/j.1365-2362.1985.tb00290.x – volume: 92 start-page: 1813 year: 2007 ident: 72672_CR32 publication-title: Biophys. J. doi: 10.1529/biophysj.106.094698 – ident: 72672_CR6 – volume: 112 start-page: 341 year: 1975 ident: 72672_CR8 publication-title: Am. Rev. Respir. Dis. doi: 10.1164/arrd.1975.112.3.341 – volume: 54 start-page: 67 year: 2018 ident: 72672_CR29 publication-title: Biorheology doi: 10.3233/BIR-17141 – volume: 10 start-page: 63 year: 2009 ident: 72672_CR20 publication-title: Respir. Res. doi: 10.1186/1465-9921-10-63 – volume: 8 start-page: 438 year: 1969 ident: 72672_CR5 publication-title: Rheol. Acta doi: 10.1007/BF01976227 – volume: 98 start-page: 380 year: 1968 ident: 72672_CR4 publication-title: Am. Rev. Respir. Dis. doi: 10.1164/arrd.1968.98.3.380 – volume: 9 start-page: e82297 year: 2014 ident: 72672_CR23 publication-title: PLoS ONE doi: 10.1371/journal.pone.0082297 – volume: 58 start-page: 1776 year: 1985 ident: 72672_CR26 publication-title: J. Appl. Physiol. doi: 10.1152/jappl.1985.58.6.1776 – volume: 18 start-page: 99 year: 2018 ident: 72672_CR19 publication-title: BMC Pulm. Med. doi: 10.1186/s12890-018-0661-1 – volume: 124 start-page: 3047 year: 2014 ident: 72672_CR21 publication-title: J. Clin. Invest. doi: 10.1172/JCI73469 – ident: 72672_CR35 – volume: 8 start-page: 2447 year: 2018 ident: 72672_CR31 publication-title: Sci. Rep. doi: 10.1038/s41598-018-20882-4 – volume: 115 start-page: 12501 year: 2018 ident: 72672_CR27 publication-title: Proc. Natl. Acad. Sci. USA doi: 10.1073/pnas.1811787115 – volume: 254 start-page: 36 year: 2018 ident: 72672_CR25 publication-title: Respir. Physiol. Neurobiol. doi: 10.1016/j.resp.2018.04.006 – volume: 156 start-page: 173 year: 1997 ident: 72672_CR17 publication-title: Am. J. Respir. Crit. Care Med. doi: 10.1164/ajrccm.156.1.9512074 – ident: 72672_CR1 – volume: 8 start-page: 381 year: 1995 ident: 72672_CR14 publication-title: Eur. Respir. J. doi: 10.1183/09031936.95.08030381 |
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| Title | Rheological analysis of sputum from patients with chronic bronchial diseases |
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