TopBP1 deficiency impairs V(D)J recombination during lymphocyte development

• Published in: The EMBO journal Vol. 33; no. 3; pp. 217 - 228
• Main Authors: Kim, Jieun, Kyu Lee, Sung, Jeon, Yoon, Kim, Yehyun, Lee, Changjin, Ho Jeon, Sung, Shim, Jaegal, Kim, In-Hoo, Hong, Seokmann, Kim, Nayoung, Lee, Ho, Seong, Rho Hyun
• Format: Journal Article
• Language: English
• Published: London Blackwell Publishing Ltd 03.02.2014; Nature Publishing Group UK; Springer Nature B.V
• Subjects: Animals; Carrier Proteins - genetics; Carrier Proteins - immunology; Carrier Proteins - metabolism; Chromatin Immunoprecipitation; Defects; Deoxyribonucleic acid; DNA; DNA - genetics; DNA Breaks, Double-Stranded; DNA Damage; DNA Repair; EMBO19; Gene Expression; Histones - genetics; Histones - metabolism; lymphocyte development; Lymphocytes; Lymphocytes - immunology; Lymphocytes - physiology; Mice; Mice, Knockout; Natural Killer T-Cells - immunology; Natural Killer T-Cells - physiology; Precursor Cells, B-Lymphoid - immunology; Precursor Cells, B-Lymphoid - physiology; Sequence Deletion; Specific Pathogen-Free Organisms; T cell receptors; TopBP1; Transgenes; V(D)J Recombination - genetics; V(D)J Recombination - immunology; VDJ recombination; VDJ recombination; TopBP1; lymphocyte development

TopBP1 was initially identified as a topoisomerase II‐β‐binding protein and it plays roles in DNA replication and repair. We found that TopBP1 is expressed at high levels in lymphoid tissues and is essential for early lymphocyte development. Specific abrogation of TopBP1 expression resulted in trans...