The impact of extraction method and pollen concentration on community composition for pollen metabarcoding

• Published in: Applications in plant sciences Vol. 12; no. 5; pp. e11601 - n/a
• Main Authors: Devriese, Arne, Peeters, Gerrit, Brys, Rein, Jacquemyn, Hans
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.09.2024; John Wiley and Sons Inc; Wiley
• Subjects: Application; bumblebees; Community composition; community structure; DNA; DNA barcoding; Ethanol; Genetic testing; Homogenization; Illumina sequencing; Introduced species; magnetism; metabarcoding; Methods; Plant extracts; Plant reproduction; plant–pollinator interactions; Pollen; Pollinators; species; Taxonomy; metabarcoding; plant–pollinator interactions; pollen; bumblebees; Illumina sequencing

Premise Plants and pollinators closely interact with each other to form complex networks of species interactions. Metabarcoding of pollen collections has recently been proposed as an advantageous method for the construction of such networks, but the extent to which diversity and community analyses d...