Optimized multiparametric flow cytometric analysis of circulating endothelial cells and their subpopulations in peripheral blood of patients with solid tumors: a technical analysis

• Published in: Cancer management and research Vol. 10; pp. 447 - 464
• Main Authors: Zhou, Fangbin, Zhou, Yaying, Yang, Ming, Wen, Jinli, Dong, Jun, Tan, Wenyong
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2018; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Age; Analysis; Angiogenesis; Antibodies; Anticoagulants; Antineoplastic agents; Biological markers; Biomarkers; Cancer metastasis; Cancer patients; Cancer research; Cancer therapies; Care and treatment; CEC Subsets; Chemotherapy; Chlorophyll; Circulating endothelial cells (CECs); Data collection; Development and progression; EDTA; Endothelium; Flow cytometry; Gene expression; Hospitals; Leukemia; Lung cancer; Medical prognosis; Medical research; Metastasis; Neovascularization; Noise; Noise control; Non-small cell lung cancer; Original Research; Patients; Prostate cancer; Proteins; Proteomics; Technical issues; Tumors; United States > US; China; flow cytometry; CEC subpopulations; CECs; methods; circulating endothelial cells

Circulating endothelial cells (CECs) and their subpopulations could be potential novel biomarkers for various malignancies. However, reliable enumerable methods are warranted to further improve their clinical utility. This study aimed to optimize a flow cytometric method (FCM) assay for CECs and sub...