Transient receptor potential vanilloid 4–expressing macrophages and keratinocytes contribute differentially to allergic and nonallergic chronic itch

• Published in: Journal of allergy and clinical immunology Vol. 141; no. 2; pp. 608 - 619.e7
• Main Authors: Luo, Jialie, Feng, Jing, Yu, Guang, Yang, Pu, Mack, Madison R., Du, Junhui, Yu, Weihua, Qian, Aihua, Zhang, Yujin, Liu, Shenbin, Yin, Shijin, Xu, Amy, Cheng, Jizhong, Liu, Qingyun, O'Neil, Roger G., Xia, Yang, Ma, Liang, Carlton, Susan M., Kim, Brian S., Renner, Kenneth, Liu, Qin, Hu, Hongzhen
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2018; Elsevier Limited
• Subjects: Allergies; Animal models; Animals; Biopsy; Calcium imaging; Cancer surgery; Chronic Disease; chronic itch; Clonal deletion; Contact dermatitis; Dendritic cells; Dermatitis; Dermatitis, Allergic Contact - genetics; Dermatitis, Allergic Contact - immunology; Dermatitis, Allergic Contact - pathology; Dermis - immunology; Dermis - pathology; Female; Flow cytometry; Gene Expression Regulation - genetics; Gene Expression Regulation - immunology; Hepatitis; Humans; Immunofluorescence; keratinocyte; Keratinocytes; Keratinocytes - immunology; Keratinocytes - pathology; Laboratories; macrophage; Macrophages; Macrophages - immunology; Macrophages - pathology; Male; Mice; Mice, Knockout; Neurons; Polymerase chain reaction; Pruritus; Pruritus - genetics; Pruritus - immunology; Pruritus - pathology; Rodents; Sensory neurons; Skin; Skin cancer; Spinal cord; Stem cells; Transient receptor potential proteins; Transient receptor potential vanilloid 4; TRPV Cation Channels - genetics; TRPV Cation Channels - immunology; ACD; eGFP; PBS+TX; [Ca2+]i; chronic itch; macrophage; DTX; SADBE; CIP; Transient receptor potential vanilloid 4; TPH; GFP; Ct; AEW; Htr7; keratinocyte; TRPV4; pCPA; DRG; Htr2a; IRB; 5-HT; GAPDH; WT

Chronic itch is a highly debilitating symptom that underlies many medical disorders with no universally effective treatments. Although unique neuronal signaling cascades in the sensory ganglia and spinal cord have been shown to critically promote the pathogenesis of chronic itch, the role of skin-as...