Critical roles of FGF, RA, and WNT signalling in the development of the human otic placode and subsequent lineages in a dish

• Published in: Regenerative therapy Vol. 20; pp. 165 - 186
• Main Authors: Saeki, Tsubasa, Yoshimatsu, Sho, Ishikawa, Mitsuru, Hon, Chung-Chau, Koya, Ikuko, Shibata, Shinsuke, Hosoya, Makoto, Saegusa, Chika, Ogawa, Kaoru, Shin, Jay W., Fujioka, Masato, Okano, Hideyuki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.06.2022; Japanese Society for Regenerative Medicine; Elsevier
• Subjects: Floating culture; Hair cell; Human pluripotent stem cell; Original; Otic placode; Single cell RNA-seq; PBS; OCT; BMP; FGF; hPSCs; EGF; Otic placode; KSR; RA; SB; Floating culture; CHIR; 3D; scRNA-seq; 2D; SF; Human pluripotent stem cell; Single cell RNA-seq; LDN; TGFβ; Hair cell; qPCR; SEM; hESCs; SB, SB-431542; EGF, epidermal growth factor; FGF, fibroblast growth factor; 3D, three-dimensional; SF, serum free; TGFβ, transforming growth factor-β; scRNA-seq, single-cell RNA-sequencing; 2D, two-dimensional; PBS, phosphate-buffered saline; hPSCs, human pluripotent stem cells; OCT, optimal cutting temperature; LDN, LDN-193189; KSR, KnockOut serum replacement; SEM, scanning electron microscope; BMP, bone morphogenetic protein; qPCR, quantitative PCR; RA, retinoic acid; hESCs, human embryonic stem cells; CHIR, CHIR-99021

Efficient induction of the otic placode, the developmental origin of the inner ear from human pluripotent stem cells (hPSCs), provides a robust platform for otic development and sensorineural hearing loss modelling. Nevertheless, there remains a limited capacity of otic lineage specification from hP...