Passive immunisation against Pseudomonas aeruginosa recombinant flagellin in an experimental model of burn wound sepsis

• Published in: Burns Vol. 37; no. 5; pp. 865 - 872
• Main Authors: Faezi, Sobhan, Sattari, Morteza, Mahdavi, Mehdi, Roudkenar, Mehryar Habibi
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2011; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - therapeutic use; Bacterial diseases; Bacterial sepsis; Biological and medical sciences; Blotting, Western; Burns; Burns - drug therapy; Burns - microbiology; Critical Care; Flagellin - immunology; Human bacterial diseases; Immunisation; Immunization, Passive; Immunoglobulin G - immunology; Infectious diseases; Medical sciences; Mice; Passive; Phagocytosis - drug effects; Prevention and actions; Pseudomonas aeruginosa; Pseudomonas aeruginosa - immunology; Pseudomonas Infections - immunology; Pseudomonas Infections - mortality; Pseudomonas Infections - prevention & control; Public health. Hygiene; Public health. Hygiene-occupational medicine; Recombinant flagellin; Recombinant Proteins - therapeutic use; Sepsis - drug therapy; Sepsis - microbiology; Sepsis - prevention & control; Skin - microbiology; Traumas. Diseases due to physical agents; Wound Infection - drug therapy; Wound Infection - microbiology; Pseudomonas aeruginosa; Passive; Recombinant flagellin; Immunisation; Pseudomonadales; Skin disease; Vaccination; Wound; Bacteremia; Infection; Burn; Prevention; Sepsis syndrome; Bacteriosis; Bacteria; Pseudomonadaceae; Models
• ISSN: 0305-4179; 1879-1409
• DOI: 10.1016/j.burns.2010.12.003

Abstract Background This study was aimed to investigate whether anti-recombinant flagellin type A (anti r-fla-A) immunoglobulin G (IgG) provides protection in a mouse burn model of infection, and to determine the role of anti r-fla-A IgG as an opsonin and motility inhibitor in local and systemic infections. Methods Following the preparation of r-flagellin type A, rabbit polyclonal IgG was prepared. Specificity of anti r-flagellin for r-flagellin was evaluated by immunoblot analysis. After burn and challenge, mortality rate was screened in the mice treated with anti r-fla-A IgG. The ability of antiserum to promote phagocytosis of bacteria was assessed by the opsonophagocytosis testing. Functional activity of anti r-fla-A IgG was assessed in vitro by motility inhibition assay. Bacterial quantity in skin and internal organs was evaluated to study systemic infection. Results In vivo administration of anti r-fla-A IgG resulted in a significant improvement in survival in mice infected by a homologous strain of Pseudomonas aeruginosa from 16.6% to 75% compared with the control IgG. By contrast, this rate was 33.3% in the mice infected by the heterologous strain, PAO1 (type B flagellated strain). Protection was improved by giving a second treatment of r-flagellin antisera at 24-h post-burn and infection. Furthermore, anti r-fla-A IgG enhanced considerably the phagocytosis of the homologous strain but it was slight in the heterologous strain. The antiserum against r-flagellin type A was able to inhibit the motility of the PAK strain (type A flagellated strain), but slight inhibition was observed against PAO1. Meanwhile, anti r-fla-A IgG inhibited the systemic spread of PAK strain from the site of infection to internal organs. Conclusion In this study, passive immunisation with anti r-fla-A IgG was active against a homologous strain of infecting P. aeruginosa , but lost most of its efficiency against a heterologous strain. Therefore, passive treatment with anti r-fla-A IgG might protect burned mice against local and systemic infection of P. aeruginosa.