How Do Gyrating Beads Accelerate Amyloid Fibrillization?

• Published in: Biophysical journal Vol. 112; no. 2; pp. 250 - 264
• Main Authors: Abdolvahabi, Alireza, Shi, Yunhua, Rasouli, Sanaz, Croom, Corbin M., Chuprin, Aleksandra, Shaw, Bryan F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 24.01.2017; Biophysical Society; The Biophysical Society
• Subjects: Amyloid - chemistry; Bioassays; Biophysics; Humans; Hydrophobic and Hydrophilic Interactions; Kinetics; Microspheres; Models, Molecular; Molecules; Mutation; Protein Multimerization - drug effects; Protein Structure, Secondary; Proteins; Rotation; Superoxide Dismutase-1 - chemistry

The chemical and physical mechanisms by which gyrating beads accelerate amyloid fibrillization in microtiter plate assays are unclear. Identifying these mechanisms will help optimize high-throughput screening assays for molecules and mutations that modulate aggregation and might explain why differen...