Long-term exposure of MCF-12A normal human breast epithelial cells to ethanol induces epithelial mesenchymal transition and oncogenic features

• Published in: International journal of oncology Vol. 48; no. 6; pp. 2399 - 2414
• Main Authors: GELFAND, ROBERT, VERNET, DOLORES, BRUHN, KEVIN, VADGAMA, JAYDUTT, GONZALEZ-CADAVID, NESTOR F
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.06.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Acetaldehyde - pharmacology; Alcohol; Apoptosis; Breast - cytology; Breast - drug effects; Breast - physiology; Breast cancer; Breast Neoplasms - genetics; Cell culture; Cell Line; Cloning; Deoxyribonucleic acid; Development and progression; DNA; DNA methylation; Dose-Response Relationship, Drug; Drinking of alcoholic beverages; Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - physiology; epithelial mesenchymal transition; Epithelial-Mesenchymal Transition - drug effects; Epithelial-Mesenchymal Transition - genetics; Estrogens; ethanol; Ethanol - pharmacology; Female; Gene amplification; Gene expression; Gene Expression Regulation - drug effects; Growth factors; Health aspects; Humans; Kinases; Laboratories; MCF-12A; MicroRNAs; MicroRNAs - genetics; MicroRNAs - metabolism; Phenotype; Properties; Proteins; Studies; Transcription, Genetic - drug effects; Transcriptome - drug effects; Tumors

Alcoholism is associated with breast cancer incidence and progression, and moderate chronic consumption of ethanol is a risk factor. The mechanisms involved in alcohol's oncogenic effects are unknown, but it has been speculated that they may be mediated by acetaldehyde. We used the immortalized...