Trans-Golgi Network-Located AP1 Gamma Adaptins Mediate Dileucine Motif-Directed Vacuolar Targeting in Arabidopsis

• Published in: The Plant cell Vol. 26; no. 10; pp. 4102 - 4118
• Main Authors: Wang, Xiangfeng, Cai, Yi, Wang, Hao, Zeng, Yonglun, Zhuang, Xiaohong, Li, Baiying, Jiang, Liwen
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Biologists 01.10.2014
• Subjects: Adaptor Protein Complex gamma Subunits - genetics; Adaptor Protein Complex gamma Subunits - metabolism; Amino Acid Motifs - genetics; Amino Acid Sequence; Amino acids; Arabidopsis; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Cation Transport Proteins - genetics; Cation Transport Proteins - metabolism; Correlation coefficients; Endoplasmic Reticulum - metabolism; Freight; Immunoblotting; Intracellular Membranes - metabolism; Leucine - genetics; Leucine - metabolism; Membrane proteins; Microscopy, Confocal; Molecular Sequence Data; Mutation; Oligopeptides - genetics; Oligopeptides - metabolism; Peptides - genetics; Peptides - metabolism; Plant cells; Plants; Plants, Genetically Modified; Protein Binding; Protein Transport; Proteins; Protoplasts; Protoplasts - metabolism; Sequence Homology, Amino Acid; Tonoplast; trans-Golgi Network - metabolism; Vacuoles; Vacuoles - metabolism

Membrane proteins on the tonoplast are indispensible for vacuolar functions in plants. However, how these proteins are transported to the vacuole and how they become separated from plasma membrane proteins remain largely unknown. In this study, we used Arabidopsis thaliana vacuolar ion transporteri...