Association of the Halobacterial 7S RNA to the Polysome Correlates with Expression of the Membrane Protein Bacterioopsin
The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These two RNAs cosedimented predominantly with membrane-bound polysomes, and the quantity of 7S RNA bound to the ribosomes was directly correlated...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 89; no. 4; pp. 1204 - 1208 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
National Academy of Sciences of the United States of America
15.02.1992
National Acad Sciences National Academy of Sciences |
Subjects | |
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Abstract | The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These two RNAs cosedimented predominantly with membrane-bound polysomes, and the quantity of 7S RNA bound to the ribosomes was directly correlated with the expression of bacterioopsin. Puromycin treatment released the 7S RNA from the polysomes, indicating that it is transiently associated with protein translation. We suggest that halobacteria contain a signal-recognition-like particle involved in translation of membrane-associated proteins. |
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AbstractList | The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These two RNAs cosedimented predominantly with membrane-bound polysomes, and the quantity of 7S RNA bound to the ribosomes was directly correlated with the expression of bacterioopsin. Puromycin treatment released the 7S RNA from the polysomes, indicating that it is transiently associated with protein translation. We suggest that halobacteria contain a signal-recognition-like particle involved in translation of membrane-associated proteins. The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. Results suggest that halobacteria contain a signal-recognition-like particle involved in translation of membrane-associated proteins. |
Author | Betlach, Mary C. Gropp, Roswitha Gropp, Felix |
AuthorAffiliation | Department of Biochemistry and Biophysics, University of California, San Francisco 94143 |
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Copyright | Copyright 1992 The National Academy of Sciences of the United States of America 1992 INIST-CNRS Copyright National Academy of Sciences Feb 15, 1992 |
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Keywords | Membrane protein Ribonucleoprotein Archaeobacteria Genetical translation Photoprotein Halobacterium halobium Bacteriorhodopsin Messenger RNA Apoproteins Molecular association Bacteria Halobacteriaceae Polysome |
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Snippet | The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients. These... The sedimentation behavior of the halobacterial 7S RNA and bacterioopsin mRNA was assessed after application of total cell lysates to sucrose gradients.... |
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SubjectTerms | Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Bacteria Bacteriorhodopsins - metabolism Biochemistry Biological and medical sciences Cell growth Fundamental and applied biological sciences. Psychology Gels Halobacterium - metabolism Membrane Glycoproteins - chemistry Membrane Glycoproteins - metabolism Membrane proteins Membrane Proteins - chemistry Membrane Proteins - metabolism Messenger RNA Molecular Sequence Data Nucleic acids P branes Polyribosomes Polyribosomes - metabolism Proteins Puromycin - pharmacology Ribonucleic acid Ribonucleoproteins - metabolism Ribosomes Ribosomes - metabolism RNA RNA, Messenger - metabolism Rna, ribonucleoproteins RNA, Ribosomal, 5S - metabolism RNA, Small Nuclear - metabolism Signal Recognition Particle Ultracentrifugation |
Title | Association of the Halobacterial 7S RNA to the Polysome Correlates with Expression of the Membrane Protein Bacterioopsin |
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