Liquid chromatography–tandem mass spectrometric assay for ponatinib and N-desmethyl ponatinib in mouse plasma

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1023-1024; pp. 24 - 29
• Main Authors: Sparidans, Rolf W., Kort, Anita, Schinkel, Alfred H., Schellens, Jan H.M., Beijnen, Jos H.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.06.2016
• Subjects: ambient temperature; Animals; Assaying; Chromatography; Chromatography, Liquid - methods; Drugs; evaporation; Female; females; Imidazoles - blood; Imidazoles - chemistry; ionization; isotope labeling; LC−MS/MS; leukemia; Limit of Detection; Linear Models; Liquid chromatography; Liquid-liquid extraction; Liquids; mass spectrometry; Metabolites; Mice; monitoring; Mouse plasma; N-desmethyl ponatinib; oral administration; pharmacokinetics; Ponatinib; Pyridazines - blood; Pyridazines - chemistry; Reproducibility of Results; reversed-phase liquid chromatography; Spectroscopy; Tandem Mass Spectrometry - methods; ALL; LLOQ; CML; DMP; BCR-ABL; N-desmethyl ponatinib; IS; CYP3A4; Ponatinib; LC−MS/MS; SRM; Liquid-liquid extraction; QC; FVB; tBME; TKI; Ph; ESI; Mouse plasma

•The first validated bioanalytical LC–MS/MS assay for ponatinib and its active metabolite.•Liquid-liquid extraction showed a selective treatment for the mouse plasma samples.•The assay has successfully been validated in the 5–5000 and 1–1000ng/ml ranges.•The assay was successfully used for a pharmac...