Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum
Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process...
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| Published in | Cells (Basel, Switzerland) Vol. 6; no. 3; p. 18 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
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03.07.2017
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| Abstract | Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba
as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism. |
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| AbstractList | Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba
as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism. Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism. Autophagy is a eukaryotic catabolic pathway that degrades and recycles cellular components to maintain homeostasis. It can target protein aggregates, superfluous biomolecular complexes, dysfunctional and damaged organelles, as well as pathogenic intracellular microbes. Autophagy is a dynamic process in which the different stages from initiation to final degradation of cargo are finely regulated. Therefore, the study of this process requires the use of a palette of techniques, which are continuously evolving and whose interpretation is not trivial. Here, we present the social amoeba Dictyostelium discoideum as a relevant model to study autophagy. Several methods have been developed based on the tracking and observation of autophagosomes by microscopy, analysis of changes in expression of autophagy genes and proteins, and examination of the autophagic flux with various techniques. In this review, we discuss the pros and cons of the currently available techniques to assess autophagy in this organism. |
| Author | Domínguez-Martín, Eunice Soldati, Thierry Coria, Roberto Cardenal-Muñoz, Elena King, Jason S Escalante, Ricardo |
| AuthorAffiliation | 1 Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain; edominguez@iib.uam.es 2 Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico; rcoria@ifc.unam.mx 4 Department of Biomedical Sciences and Bateson Centre, University of Sheffield, Sheffield S10 2TN, UK; jason.king@sheffield.ac.uk 3 Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland; Elena.Cardenal@unige.ch (E.C.-M.); Thierry.Soldati@unige.ch (T.S.) |
| AuthorAffiliation_xml | – name: 3 Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland; Elena.Cardenal@unige.ch (E.C.-M.); Thierry.Soldati@unige.ch (T.S.) – name: 4 Department of Biomedical Sciences and Bateson Centre, University of Sheffield, Sheffield S10 2TN, UK; jason.king@sheffield.ac.uk – name: 2 Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico; rcoria@ifc.unam.mx – name: 1 Instituto de Investigaciones Biomédicas “Alberto Sols” (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain; edominguez@iib.uam.es |
| Author_xml | – sequence: 1 givenname: Eunice surname: Domínguez-Martín fullname: Domínguez-Martín, Eunice email: edominguez@iib.uam.es, edominguez@iib.uam.es organization: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico. edominguez@iib.uam.es – sequence: 2 givenname: Elena surname: Cardenal-Muñoz fullname: Cardenal-Muñoz, Elena email: Elena.Cardenal@unige.ch organization: Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland. Elena.Cardenal@unige.ch – sequence: 3 givenname: Jason S surname: King fullname: King, Jason S email: jason.king@sheffield.ac.uk organization: Department of Biomedical Sciences and Bateson Centre, University of Sheffield, Sheffield S10 2TN, UK. jason.king@sheffield.ac.uk – sequence: 4 givenname: Thierry surname: Soldati fullname: Soldati, Thierry email: Thierry.Soldati@unige.ch organization: Département de Biochimie, Faculté des Sciences, Université de Genève, Sciences II, CH-1211-Genève-4, Switzerland. Thierry.Soldati@unige.ch – sequence: 5 givenname: Roberto surname: Coria fullname: Coria, Roberto email: rcoria@ifc.unam.mx organization: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, 04510 Ciudad de México, Mexico. rcoria@ifc.unam.mx – sequence: 6 givenname: Ricardo surname: Escalante fullname: Escalante, Ricardo email: rescalante@iib.uam.es organization: Instituto de Investigaciones Biomédicas "Alberto Sols" (CSIC-UAM), Arturo Duperier 4, 28029 Madrid, Spain. rescalante@iib.uam.es |
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| Keywords | Dictyostelium autophagy autophagic markers cleavage assays flux assays |
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| SubjectTerms | Amoeba autophagic markers Autophagy cleavage assays Dictyostelium flux assays Homeostasis Organelles Phagocytosis Phagosomes Review |
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| Title | Methods to Monitor and Quantify Autophagy in the Social Amoeba Dictyostelium discoideum |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/28671610 https://www.proquest.com/docview/1952648280 https://search.proquest.com/docview/1915558991 https://pubmed.ncbi.nlm.nih.gov/PMC5617964 https://doaj.org/article/e0c78647c4da4163a53df63c07f974e6 |
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