Arabidopsis mutants deficient in T-DNA integration

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 92; no. 25; pp. 11786 - 11790
• Main Authors: Sonti, R.V. (Center for Cellular and Molecular Biology, Hyderabad, India.), Chiurazzi, M, Wong, D, Davies, C.S, Harlow, G.R, Mount, D.W, Signer, E.R
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 05.12.1995; National Acad Sciences; National Academy of Sciences
• Subjects: ADN; Agrobacterium; AGROBACTERIUM TUMEFACIENS; Agrobacterium tumefaciens - genetics; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis - radiation effects; ARABIDOPSIS THALIANA; Bacteria; Culture Techniques; Deoxyribonucleic acid; DNA; DNA Repair; DNA, Bacterial - genetics; GENE; GENES; Genetic mutation; Genetic vectors; Genetics; Genomics; GLICOSIDASAS; GLYCOSIDASE; Hypersensitivity; MUTANT; MUTANTES; Mutation; Plant cells; Plant roots; Plant Roots - genetics; Plant Roots - growth & development; Plant Shoots - genetics; Plant Shoots - growth & development; Plants; Plasmids; RACINE; RADIACION GAMMA; RADIACION ULTRAVIOLETA; Radiation Tolerance; RAICES; RAYONNEMENT GAMMA; RAYONNEMENT ULTRAVIOLET; Space life sciences; TRANSFORMACION GENETICA; TRANSFORMATION GENETIQUE; Transformation, Genetic; Ultraviolet radiation; Ultraviolet Rays - adverse effects
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.92.25.11786

Arabidopsis thaliana mutants originally isolated as hypersensitive to irradiation were screened for the ability to be transformed by Agrobacterium transferred DNA (T-DNA). One of four UV-hypersensitive mutants and one of two gamma-hypersensitive mutants tested showed a significant reduction in the frequency of stable transformants compared with radioresistant controls. In a transient assay for T-DNA transfer independent of genomic integration, both mutant lines took up and expressed T-DNA as efficiently as parental lines. These lines are therefore deficient specifically in stable T-DNA integration and thus provide direct evidence for the role of a plant function in that process. As radiation hypersensitivity suggests a deficiency in repair of DNA damage, that plant function may be one that is also involved in DNA repair, possibly, from other evidence, in repair of double-strand DNA breaks