The Statistical Conformation of a Highly Flexible Protein: Small-Angle X-Ray Scattering of S. aureus Protein A

• Published in: Structure (London) Vol. 22; no. 8; pp. 1184 - 1195
• Main Authors: Capp, Jo A., Hagarman, Andrew, Richardson, David C., Oas, Terrence G.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 05.08.2014; Elsevier
• Subjects: Amino Acid Sequence; Binding; Mathematical analysis; Mathematical models; Models, Molecular; Molecular Biology - methods; Molecular Sequence Data; Polymer physics; Protein A; Protein Conformation; Proteins; SAXS; Scattering functions; Scattering, Small Angle; Staphylococcal Protein A - chemistry; Staphylococcus aureus - chemistry

Staphylococcal protein A (SpA) is a multidomain protein consisting of five globular IgG binding domains separated by a conserved six- to nine-residue flexible linker. We collected SAXS data on the N-terminal protein-binding half of SpA (SpA-N) and constructs consisting of one to five domain modules...