Biochemical and structural characterization of a sphingomonad diarylpropane lyase for cofactorless deformylation

• Published in: Proceedings of the National Academy of Sciences Vol. 120; no. 4; p. e2212246120
• Main Authors: Kuatsjah, Eugene, Zahn, Michael, Chen, Xiangyang, Kato, Ryo, Hinchen, Daniel J., Konev, Mikhail O., Katahira, Rui, Orr, Christian, Wagner, Armin, Zou, Yike, Haugen, Stefan J., Ramirez, Kelsey J., Michener, Joshua K., Pickford, Andrew R., Kamimura, Naofumi, Masai, Eiji, Houk, K. N., McGeehan, John E., Beckham, Gregg T.
• Format: Journal Article
• Language: English
• Published: United States Proceedings of the National Academy of Sciences 24.01.2023; National Academy of Sciences
• Subjects: 09 BIOMASS FUELS; aromatic catabolism; Bacterial Proteins; Bacterial Proteins - metabolism; BASIC BIOLOGICAL SCIENCES; Biological Sciences; Carbon; Catabolism; Covalent bonds; Crystallography; Deformation; Density functional theory; Depolymerization; Dimers; Enantiomers; Enzymes; INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Lignin; Lignin - metabolism; Lyases; Monomers; Novosphingobium aromaticivorans; NTF-2; Nuclear transport; Oxidoreductases; Oxidoreductases - metabolism; Physical Sciences; Product inhibition; Quinones; Reaction products; Sphingobium sp. SYK-6; Stereoisomerism; Structural analysis; Substrates; Syk protein; X-ray crystallography; Novosphingobium aromaticivorans; lignin; Sphingobium sp. SYK-6; aromatic catabolism; NTF-2
• ISSN: 0027-8424; 1091-6490; 1091-6490
• DOI: 10.1073/pnas.2212246120

Lignin valorization is being intensely pursued via tandem catalytic depolymerization and biological funneling to produce single products. In many lignin depolymerization processes, aromatic dimers and oligomers linked by carbon–carbon bonds remain intact, necessitating the development of enzymes cap...