The role of plasmalemmal-cortical anchoring on the stability of transmembrane electropores

• Published in: IEEE transactions on dielectrics and electrical insulation Vol. 16; no. 5; pp. 1251 - 1258
• Main Authors: Kennedy, S., Ji, Z., Rockweiler, N., Hahn, A., Booske, J., Hagness, S.
• Format: Journal Article
• Language: English
• Published: United States IEEE 01.10.2009; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: actin cortex; Anchoring; Biomembranes; Brain modeling; Constraints; Density; Dynamics; Electroporation; gene and drug delivery; Manipulator dynamics; Mathematical models; Membranes; Plasma density; Plasma properties; Plasma stability; Predictive models; Proteins; Pulsed electric fields; Stability; Surface tension; Wounds
• ISSN: 1070-9878; 1558-4135
• DOI: 10.1109/TDEI.2009.5293935

The structure of eukaryotic cells is maintained by a network of filamentous actin anchored subjacently to the plasma membrane. This structure is referred to as the actin cortex. We present a locally constrained surface tension model for electroporation in order to address the influence of plasmalemmal-cortical anchoring on electropore dynamics. This model predicts that stable electropores are possible under certain conditions. The existence of stable electropores has been suggested in several experimental studies. The electropore radius at which stability is achieved is a function of the characteristic radii of locally constrained regions about the plasma membrane. This model opens the possibility of using actin-modifying compounds to physically manipulate cortical density, thereby manipulating electroporation dynamics. It also underscores the need to improve electroporation models further by incorporating the influence of trans-electropore ionic and aqueous flow, cortical flexibility, transmembrane protein mobility, and active cellular wound healing mechanisms.