Total ApoE and ApoE4 Isoform Assays in an Alzheimer's Disease Case-control Study by Targeted Mass Spectrometry (n = 669): A Pilot Assay for Methionine-containing Proteotypic Peptides

• Published in: Molecular & cellular proteomics Vol. 11; no. 11; pp. 1389 - 1403
• Main Authors: Simon, Romain, Girod, Marion, Fonbonne, Catherine, Salvador, Arnaud, Clément, Yohann, Lantéri, Pierre, Amouyel, Philippe, Lambert, Jean Charles, Lemoine, Jérôme
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2012; American Society for Biochemistry and Molecular Biology; The American Society for Biochemistry and Molecular Biology
• Subjects: Alzheimer Disease - blood; Alzheimer Disease - metabolism; Amino Acid Sequence; Analytical chemistry; Apolipoprotein E4 - blood; Apolipoprotein E4 - chemistry; Apolipoprotein E4 - metabolism; Biochemistry; Biochemistry, Molecular Biology; Calibration; Case-Control Studies; Chemical Sciences; Cohort Studies; Humans; Hydrolysis; Life Sciences; Mass Spectrometry - methods; Methionine - metabolism; Molecular Sequence Data; Oxidation-Reduction; Peptides - blood; Peptides - chemistry; Peptides - metabolism; Pilot Projects; Principal Component Analysis; Protein Isoforms - chemistry; Protein Isoforms - metabolism; Reproducibility of Results; Time Factors; E GENE; MULTIPLEXED ASSAYS; LINKAGE DISEQUILIBRIUM; QUANTITATIVE PROTEOMICS; BIOMARKER DISCOVERY; REGULATORY REGION; APOLIPOPROTEIN-E APOE; PROMOTER POLYMORPHISM; ABUNDANCE PROTEINS; CEREBROSPINAL-FLUID

Allelic polymorphism of the apolipoprotein E (ApoE) gene (ApoE ε2, ApoE ε3 and ApoE ε4 alleles) gives rise to three protein isoforms (ApoE2, ApoE3 and ApoE4) that differ by 1 or 2 amino acids. Inheritance of the ApoE ε4 allele is a risk factor for developing Alzheimer's disease (AD). The potent...