Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov., isolated from human stomach mucosa

• Published in: International journal of systematic and evolutionary microbiology Vol. 55; no. 1; pp. 77 - 82
• Main Authors: Roos, Stefan, Engstrand, Lars, Jonsson, Hans
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2005; Society for General Microbiology
• Subjects: Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; DNA, Ribosomal - analysis; Fundamental and applied biological sciences. Psychology; Gastric Mucosa - microbiology; Genes, rRNA; Humans; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Lactobacillus/classification/genetics/isolation & purification; Medicin och hälsovetenskap; Microbiology; Molecular Sequence Data; Phenotype; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Systematics; Human; Stomach; Lactic acid bacteria; Digestive system; Taxonomy; Mucosa; Bacteria; Lactobacillaceae; New species; Lactobacillus; Research Support; RNA; Ribosomal; DNA; Genes; rRNA; Ribosomal/analysis; Sequence Analysis; 16S/genetics; Non-U.S. Gov't
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.63083-0

1 Department of Microbiology, Swedish University of Agricultural Sciences, Box 7025, SE-750 07 Uppsala, Sweden 2 Swedish Institute for Infectious Disease Control, SE-171 82 Solna, Sweden Correspondence Stefan Roos stefan.roos{at}mikrob.slu.se In an attempt to study the composition of the Lactobacillus flora from mucosa of human stomach, 16S rRNA gene sequences of 129 isolates were analysed. For 15 of these, the results differed significantly from known sequences, and additional tests were performed to determine whether these isolates represented as yet unrecognized species. Phenotypic and genetic characteristics revealed that these isolates represented four novel Lactobacillus species. Two belong to the Lactobacillus reuteri and the other two to the Lactobacillus delbrueckii subgroup of Lactobacillus . The names Lactobacillus gastricus sp. nov., Lactobacillus antri sp. nov., Lactobacillus kalixensis sp. nov. and Lactobacillus ultunensis sp. nov. are proposed, with the respective type strains Kx156A7 T (=LMG 22113 T =DSM 16045 T =CCUG 48454 T ), Kx146A4 T (=LMG 22111 T =DSM 16041 T =CCUG 48456 T ), Kx127A2 T (=LMG 22115 T =DSM 16043 T =CCUG 48459 T ) and Kx146C1 T (=LMG 22117 T =DSM 16047 T =CCUG 48460 T ). Published online ahead of print on 20 August 2004 as DOI 10.1099/ijs.0.63083-0. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of Lactobacillus gastricus Kx156A7 T , Lactobacillus antri Kx146A4 T , Lactobacillus kalixensis Kx127A2 T and Lactobacillus ultunensis Kx146C1 T are AY253658 , AY253659 , AY253657 and AY253660 , respectively.