The novel sRNA s015 improves nisin yield by increasing acid tolerance of Lactococcus lactis F44

• Published in: Applied microbiology and biotechnology Vol. 101; no. 16; pp. 6483 - 6493
• Main Authors: Qi, Jiakun, Caiyin, Qinggele, Wu, Hao, Tian, Kairen, Wang, Binbin, Li, Yanni, Qiao, Jianjun
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2017; Springer; Springer Nature B.V
• Subjects: acid tolerance; Acids; Adaptation, Physiological - genetics; Anti-Bacterial Agents - biosynthesis; Antimicrobial agents; antimicrobial peptides; Applied Microbial and Cell Physiology; Bacteria; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Computer Simulation; Gene sequencing; Genetic aspects; Hydrogen-Ion Concentration; Lactococcus; Lactococcus lactis; Lactococcus lactis - drug effects; Lactococcus lactis - genetics; Lactococcus lactis - metabolism; Lactococcus lactis - physiology; Libraries; Life Sciences; messenger RNA; Methods; Microbial Genetics and Genomics; Microbiology; Nisin; Nisin - analysis; Nisin - biosynthesis; non-coding RNA; Northern blotting; Peptides; Physiological aspects; Polymerase chain reaction; Post-transcription; prediction; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction; reverse transcriptase polymerase chain reaction; Ribonucleic acid; RNA; RNA sequencing; RNA, Small Untranslated - genetics; sequence analysis; Sequence Analysis, RNA; Statistical analysis; Transcription; Nisin; Acid tolerance; sRNA; L. lactis

Nisin, a polycyclic antibacterial peptide produced by Lactococcus lactis , is stable at low pH. Improving the acid tolerance of L. lactis could thus enhance nisin yield. Small non-coding RNAs (sRNAs) play essential roles in acid tolerance by regulating their target mRNAs at the post-transcriptional...