The applications of CRISPR/Cas system in molecular detection

• Published in: Journal of Cellular and Molecular Medicine Vol. 22; no. 12; pp. 5807 - 5815
• Main Authors: Zhou, Li, Peng, Rongxue, Zhang, Rui, Li, Jinming
• Format: Journal Article
• Language: English
• Published: England Wiley 01.12.2018; John Wiley & Sons, Inc; John Wiley and Sons Inc
• Subjects: Bacteria; Bacteria - isolation & purification; Base Sequence; CRISPR; CRISPR-Cas Systems; CRISPR-Cas Systems - genetics; Deoxyribonucleic acid; diagnostics; DNA; Genome editing; molecular detection; Molecular Diagnostic Techniques; Mutation; Mutation - genetics; Nucleotide sequence; Pathogens; Polymorphism, Single Nucleotide; Polymorphism, Single Nucleotide - genetics; Review; Reviews; Ribonucleic acid; RNA; single nucleotide variants; Streptococcus infections; Viruses; Viruses - isolation & purification; diagnostics; single nucleotide variants; molecular detection; pathogens; CRISPR/Cas systems

The Streptococcus pyogenes CRISPR/Cas system has found widespread applications as a gene‐editing and regulatory tool for the simultaneous delivery of the Cas9 protein and guide RNAs into the cell, thus making the recognition of specific DNA sequences possible. The recent study that shows that Cas9 c...