Cryo‐EM structure of the Ebola virus nucleoprotein–RNA complex

• Published in: Acta crystallographica. Section F, Structural biology communications Vol. 75; no. 5; pp. 340 - 347
• Main Authors: Kirchdoerfer, Robert N., Saphire, Erica Ollmann, Ward, Andrew B.
• Format: Journal Article
• Language: English
• Published: 5 Abbey Square, Chester, Cheshire CH1 2HU, England International Union of Crystallography 01.05.2019; Wiley Subscription Services, Inc
• Subjects: Amino Acid Sequence; Binding Sites; Cloning, Molecular; Cryoelectron Microscopy; cryo‐electron microscopy; Ebola virus; Ebolavirus; Ebolavirus - chemistry; Electron microscopy; Filaments; Gene Expression; Genetic Vectors - chemistry; Genetic Vectors - metabolism; Genomes; HEK293 Cells; helix; Humans; Models, Molecular; Nucleic Acid Conformation; Nucleocapsid - chemistry; Nucleocapsid - ultrastructure; Nucleocapsids; nucleoprotein; Nucleoproteins - chemistry; Nucleoproteins - genetics; Nucleoproteins - metabolism; Packaging; Protein Binding; Protein Conformation, alpha-Helical; Protein Interaction Domains and Motifs; Proteins; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Research Communications; Ribonucleic acid; RNA; RNA viruses; RNA, Viral - chemistry; RNA, Viral - genetics; RNA, Viral - metabolism; Sequence Alignment; Sequence Homology, Amino Acid; Transcription; Viral diseases; Viral Proteins - chemistry; Viral Proteins - genetics; Viral Proteins - metabolism; Ebola virus; nucleoprotein; NP; helix; cryo-electron microscopy

Ebola virus is an emerging virus that is capable of causing a deadly disease in humans. Replication, transcription and packaging of the viral genome are carried out by the viral nucleocapsid. The nucleocapsid is a complex of the viral nucleoprotein, RNA and several other viral proteins. The nucleopr...