Rim15-dependent activation of Hsf1 and Msn2/4 transcription factors by direct phosphorylation in Saccharomyces cerevisiae

• Published in: FEBS letters Vol. 587; no. 22; pp. 3648 - 3655
• Main Authors: Lee, Peter, Kim, Myung Sup, Paik, Sang-Min, Choi, Seung-Hwan, Cho, Bo-Ram, Hahn, Ji-Sook
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.11.2013
• Subjects: Amino Acid Transport Systems - genetics; Amino Acid Transport Systems - metabolism; DNA-Binding Proteins - metabolism; Gene Expression Regulation, Fungal; Heat-Shock Proteins - metabolism; Histone Demethylases - metabolism; Hsf1; Intracellular Signaling Peptides and Proteins - metabolism; Msn2/4; Phosphorylation; PKA; Protein Kinases - metabolism; Protein Processing, Post-Translational; Protein-Serine-Threonine Kinases - metabolism; Rim15; RNA Stability; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Transcription Factors - metabolism; Transcriptional Activation; Phosphorylation; Msn2/4; Hsf1; Rim15; PKA

•Rim15 activates Hsf1 target genes upon glucose starvation.•Rim15 phosphorylates Hsf1 in vitro.•Igo1 and Igo2 stabilize the transcripts of Hsf1 target genes upon glucose starvation.•Rim15 phosphorylates Msn2, but not Gis1, in vitro.•Rim15 and Yak1 phosphorylate different sites of Hsf1 and Msn2. Rim1...