The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies

• Published in: Nature protocols Vol. 16; no. 4; pp. 1966 - 1994
• Main Authors: Shanmuganathan, Meera, Kroezen, Zachary, Gill, Biban, Azab, Sandi, de Souza, Russell J., Teo, Koon K., Atkinson, Stephanie, Subbarao, Padmaja, Desai, Dipika, Anand, Sonia S., Britz-McKibbin, Philip
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.04.2021; Nature Publishing Group
• Subjects: 631/1647/320; 639/638/11/296; Analytical Chemistry; Biological Techniques; Biomedical and Life Sciences; Blood Glucose - metabolism; Calibration; Canada; Capillary electrophoresis; Cohort Studies; Computational Biology/Bioinformatics; Control charts; Electrophoresis; Electrophoresis, Capillary - methods; Electrophoresis, Capillary - standards; Epidemiologic methods; Epidemiologic Studies; Epidemiology; Fasting - blood; Female; High-Throughput Screening Assays - methods; Humans; Injection; Ions; Life Sciences; Mass Spectrometry; Mass spectroscopy; Mean; Medical examination; Metabolites; Metabolome; Metabolomics; Methods; Microarrays; Organic Chemistry; Pregnancy; Pregnant women; Preventive maintenance; Principal Component Analysis; Protocol; Quality Control; Reference Standards; Reference Values; Robust control; Scientific imaging; Serum - metabolism; Spectroscopy; Workflow; Canada

A standardized data workflow is described for large-scale serum metabolomic studies using multisegment injection-capillary electrophoresis-mass spectrometry. Multiplexed separations increase throughput (<4 min/sample) for quantitative determination of 66 polar/ionic metabolites in serum filtrates...