An ultrapotent synthetic nanobody neutralizes SARS-CoV-2 by stabilizing inactive Spike

• Published in: Science (American Association for the Advancement of Science) Vol. 370; no. 6523; pp. 1473 - 1479
• Main Authors: Schoof, Michael, Faust, Bryan, Saunders, Reuben A., Sangwan, Smriti, Rezelj, Veronica, Hoppe, Nick, Boone, Morgane, Billesbølle, Christian B., Puchades, Cristina, Azumaya, Caleigh M., Kratochvil, Huong T., Zimanyi, Marcell, Deshpande, Ishan, Liang, Jiahao, Dickinson, Sasha, Nguyen, Henry C., Chio, Cynthia M., Merz, Gregory E., Thompson, Michael C., Diwanji, Devan, Schaefer, Kaitlin, Anand, Aditya A., Dobzinski, Niv, Zha, Beth Shoshana, Simoneau, Camille R., Leon, Kristoffer, White, Kris M., Chio, Un Seng, Gupta, Meghna, Jin, Mingliang, Li, Fei, Liu, Yanxin, Zhang, Kaihua, Bulkley, David, Sun, Ming, Smith, Amber M., Rizo, Alexandrea N., Moss, Frank, Brilot, Axel F., Pourmal, Sergei, Trenker, Raphael, Pospiech, Thomas, Gupta, Sayan, Barsi-Rhyne, Benjamin, Belyy, Vladislav, Barile-Hill, Andrew W., Nock, Silke, Liu, Yuwei, Krogan, Nevan J., Ralston, Corie Y., Swaney, Danielle L., García-Sastre, Adolfo, Ott, Melanie, Vignuzzi, Marco, Walter, Peter, Manglik, Aashish, Braxton, Julian R., Lopez, Kyle E., Melo, Arthur, Paulino, Joana, Pospiech, Thomas H., Thomas, Paul V., Wang, Feng, Yu, Zanlin, Dickinson, Miles Sasha, Asarnow, Daniel, Campbell, Melody G., Li, Junrui, Tsui, Tsz Kin Martin, Trinidad, Donovan, Tse, Eric, Zhou, Fengbo, Herrera, Nadia, Schulze-Gahmen, Ursula, Thompson, Michael C., Young, Iris D., Biel, Justin
• Format: Journal Article
• Language: English
• Published: United States The American Association for the Advancement of Science 18.12.2020; AAAS; American Association for the Advancement of Science
• Subjects: ACE2; Aerosols; Affinity; Angiotensin-converting enzyme 2; Angiotensin-Converting Enzyme 2 - chemistry; Angiotensin-Converting Enzyme 2 - immunology; Animals; Antibodies, Neutralizing - chemistry; Antibodies, Neutralizing - immunology; Antibodies, Viral - chemistry; Antibodies, Viral - immunology; Antibody Affinity; Binding; Biochem; Chlorocebus aethiops; Coronaviridae; Coronaviruses; COVID-19; Cryoelectron Microscopy; Domains; Heat treatment; Humans; Microbio; Monoclonal antibodies; Nanobodies; Neutralization; Neutralization Tests; Problem Solving; Protein Binding; Protein Stability; Proteins; Receptors; Respiratory diseases; Severe acute respiratory syndrome coronavirus 2; Single-Domain Antibodies - chemistry; Single-Domain Antibodies - immunology; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - immunology; Vero Cells; Viral diseases; Yeast

Monoclonal antibodies that bind to the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) show therapeutic promise but must be produced in mammalian cells and need to be delivered intravenously. By contrast, single-domain antibodies called nanobodies can be produced in bac...