Kinetic Analysis of the Ku-DNA Binding Activity Reveals a Redox-dependent Alteration in Protein Structure That Stimulates Dissociation of the Ku-DNA Complex

• Published in: The Journal of biological chemistry Vol. 281; no. 19; pp. 13596 - 13603
• Main Authors: Andrews, Brooke J., Lehman, Jason A., Turchi, John J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 12.05.2006; American Society for Biochemistry and Molecular Biology
• Subjects: DNA - metabolism; DNA Helicases - metabolism; Humans; Kinetics; Ku Autoantigen; Oxidation-Reduction; Protein Binding; Protein Conformation

Ku is a heterodimeric protein comprising 70- and 80-kDa subunits that participate in the non-homologous end-joining (NHEJ) repair pathway for rejoining DNA double strand breaks. We have analyzed the pre-steady state binding of Ku with various DNA duplex substrates and identified a redox-sensitive Ku...