Entirely plasmid-based reverse genetics system for rotaviruses

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 114; no. 9; pp. 2349 - 2354
• Main Authors: Kanai, Yuta, Komoto, Satoshi, Kawagishi, Takahiro, Nouda, Ryotaro, Nagasawa, Naoko, Onishi, Misa, Matsuura, Yoshiharu, Taniguchi, Koki, Kobayashi, Takeshi
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28.02.2017
• Series: From the Cover
• Subjects: Animals; Base Sequence; Biological Sciences; Cell Line; Cell Line, Tumor; Cricetulus; DNA, Complementary - genetics; DNA, Complementary - metabolism; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - metabolism; Epithelial Cells - virology; Gene Expression Regulation; Genes, Reporter; Genetics; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Immunity (Disease); Luciferases - genetics; Luciferases - metabolism; Methyltransferases - genetics; Methyltransferases - metabolism; Microbiology; Multienzyme Complexes - genetics; Multienzyme Complexes - metabolism; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Orthoreovirus - genetics; Orthoreovirus - metabolism; Orthoreovirus, Mammalian - genetics; Orthoreovirus, Mammalian - metabolism; Pathogenesis; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Plasmids; Plasmids - chemistry; Plasmids - metabolism; Reverse Genetics - methods; SEE COMMENTARY; Transduction, Genetic; Vaccinia virus; Vaccinia virus - genetics; Vaccinia virus - metabolism; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; Viruses; reporter virus; reverse genetics; vaccine; rotavirus

Rotaviruses (RVs) are highly important pathogens that cause severe diarrhea among infants and young children worldwide. The understanding of the molecular mechanisms underlying RV replication and pathogenesis has been hampered by the lack of an entirely plasmid-based reverse genetics system. In this...