Entirely plasmid-based reverse genetics system for rotaviruses - Kanai, Yuta; Komoto, Satoshi; Kawagishi, Takahiro; Nouda, Ryotaro; Nagasawa, Naoko; Onishi, Misa; Matsuura, Yoshiharu; Taniguchi, Koki; Kobayashi, Takeshi (Proceedings of the National Academy of Sciences - PNAS, 2017)
Keywords: Animals; Base Sequence; Biological Sciences; Cell Line; Cell Line, Tumor; Cricetulus; DNA, Complementary - genetics; DNA, Complementary - metabolism; DNA-Directed RNA Polymerases - genetics; DNA-Directed RNA Polymerases - metabolism; Epithelial Cells - virology; Gene Expression Regulation; Genes, Reporter; Genetics; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Humans; Immunity (Disease); Luciferases - genetics; Luciferases - metabolism; Methyltransferases - genetics; Methyltransferases - metabolism; Microbiology; Multienzyme Complexes - genetics; Multienzyme Complexes - metabolism; Nucleotidyltransferases - genetics; Nucleotidyltransferases - metabolism; Orthoreovirus - genetics; Orthoreovirus - metabolism; Orthoreovirus, Mammalian - genetics; Orthoreovirus, Mammalian - metabolism; Pathogenesis; Phosphoric Monoester Hydrolases - genetics; Phosphoric Monoester Hydrolases - metabolism; Plasmids; Plasmids - chemistry; Plasmids - metabolism; Reverse Genetics - methods; SEE COMMENTARY; Transduction, Genetic; Vaccinia virus; Vaccinia virus - genetics; Vaccinia virus - metabolism; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; Viruses; reporter virus; reverse genetics; vaccine; rotavirus