Noninvasive method for monitoring Pneumocystis carinii pneumonia

The progression of Pneumocystis carinii pneumonia was temporally monitored and quantified by real-time polymerase chain reaction of P. carinii-specific DNA in oral swabs and lung homogenates from infected rats. DNA levels correlated with the number of P. carinii organisms in the rats' lungs, as...

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Published inEmerging infectious diseases Vol. 9; no. 12; pp. 1613 - 1616
Main Authors Linke, Michael J, Rebholz, Sandy, Collins, Margaret, Tanaka, Reiko, Cushion, Melanie T
Format Journal Article
LanguageEnglish
Published United States U.S. National Center for Infectious Diseases 01.12.2003
Centers for Disease Control and Prevention
Subjects
Animals
Bacterial pneumonia
bronchoalveolar lavage
Dispatch
DNA, Fungal - chemistry
DNA, Fungal - genetics
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
fungal
Immunocompromised Host
Lung - microbiology
lung diseases
Male
Mouth Mucosa - microbiology
Pneumocystis carinii
Pneumocystis carinii - genetics
Pneumocystis carinii - isolation & purification
Pneumonia
Pneumonia, Pneumocystis - diagnosis
Pneumonia, Pneumocystis - microbiology
polymerase chain reaction
Polymerase Chain Reaction - methods
Rats
Rats, Long-Evans
Statistics, Nonparametric
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Summary:The progression of Pneumocystis carinii pneumonia was temporally monitored and quantified by real-time polymerase chain reaction of P. carinii-specific DNA in oral swabs and lung homogenates from infected rats. DNA levels correlated with the number of P. carinii organisms in the rats' lungs, as enumerated by microscopic methods. This report is the first of a noninvasive, antemortem method that can be used to monitor infection in a host over time.
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ISSN:1080-6040
1080-6059
DOI:10.3201/eid0912.030270