Alginate production by clinical nonmucoid Pseudomonas aeruginosa strains
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| Published in | Journal of Clinical Microbiology Vol. 25; no. 4; pp. 656 - 659 |
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| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
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Washington, DC
American Society for Microbiology
01.04.1987
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The slime material from a revertant nonmucoid variant, derived by serial passage of a heavily mucoid Pseudomonas aeruginosa strain isolated from a patient with bacteremia, was found to contain 16% uronic acids, 48.5% carbohydrates, 11% protein, and 2% lipids. Chromatographic analysis by ion exchange chromatography revealed that this extracellular material consisted of three fractions, one uronic acid fraction with properties similar to those of the alginate fraction of the parental strain and two other fractions consisting of neutral sugars and proteins in approximately a 5:1 ratio. In addition, the slime material from six other clinical macroscopic nonmucoid P. aeruginosa strains was found to contain alginate. These results demonstrate that alginate production in various amounts is a property shared by all P. aeruginosa strains. The slime material from a revertant nonmucoid variant, derived by serial passage of a heavily mucoid Pseudomonas aeruginosa strain isolated from a patient with bacteremia, was found to contain 16% uronic acids, 48.5% carbohydrates, 11% protein, and 2% lipids. Chromatography analysis by ion exchange chromatography revealed that this extracellular material consisted of three fractions, one uronic acid fraction with properties similar to those of the alginate fraction of the parental strain and two other fractions consisting of neutral sugars and proteins in approximately a 5:1 ratio. In addition, the slime material from six other clinical macroscopic nonmucoid P. aeruginosa strains was found to contain alginate. These results demonstrate that alginate production in various amounts is a property shared by all P. aeruginosa strains. |
| Author | A C Mintzas E D Anastassiou G Dimitracopoulos C Kounavis |
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| Keywords | Pseudomonadales Mucus Alginates Mucoid form Extracellular Bacteremia Strain Infection Variant Reversión Bacteriosis Bacteria Pseudomonadaceae Pseudomonas aeruginosa Chemical composition Clinical isolate Comparative study |
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| References | 106008 - Infect Immun. 1979 Jan;23(1):87-93 4204237 - J Infect Dis. 1974 Feb;129(2):101-9 4922568 - J Gen Microbiol. 1970 Mar;60(3):373-80 16557875 - Infect Immun. 1970 Nov;2(5):543-8 6220094 - J Infect Dis. 1983 Mar;147(3):494-503 14907713 - J Biol Chem. 1951 Nov;193(1):265-75 3919114 - J Infect Dis. 1985 Apr;151(4):581-8 3084789 - J Med Microbiol. 1986 May;21(3):199-202 3096627 - Diagn Microbiol Infect Dis. 1986 Nov;5(4):277-83 13971270 - Anal Biochem. 1962 Oct;4:330-4 6790439 - Infect Immun. 1981 Jul;33(1):142-8 14222269 - Nature. 1964 Oct 10;204:187-8 14908032 - J Gen Microbiol. 1951 Nov;5(5 Suppl.):939-50 4240755 - Biochem J. 1969 May;112(4):521-5 812998 - J Med Microbiol. 1975 Nov;8(4):513-22 812335 - Acta Pathol Microbiol Scand Suppl. 1975 Dec;83(6):553-60 809484 - J Clin Microbiol. 1975 Jun;1(6):521-6 5004935 - Tex Rep Biol Med. 1971 Fall;29(3):265-72 6793519 - Infect Immun. 1981 Sep;33(3):788-94 4984207 - Appl Microbiol. 1969 Nov;18(5):936-7 6772562 - Infect Immun. 1980 May;28(2):546-56 4099895 - Lancet. 1971 Jan 30;1(7692):236-7 411754 - Infect Immun. 1977 Nov;18(2):304-9 96088 - J Bacteriol. 1978 May;134(2):418-22 14067096 - Proc Natl Acad Sci U S A. 1963 Sep;50:499-506 13059016 - Biochim Biophys Acta. 1953 Apr;10(4):512-23 |
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Mendeley... The slime material from a revertant nonmucoid variant, derived by serial passage of a heavily mucoid Pseudomonas aeruginosa strain isolated from a patient with... |
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| SubjectTerms | Alginates - analysis Alginates - biosynthesis Bacteriology Biological and medical sciences Chromatography, Ion Exchange Chromatography, Thin Layer Fundamental and applied biological sciences. Psychology Humans Metabolism. Enzymes Microbiology Pseudomonas aeruginosa Pseudomonas aeruginosa - metabolism |
| Title | Alginate production by clinical nonmucoid Pseudomonas aeruginosa strains |
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