Monitoring local delivery of vancomycin from gelatin nanospheres in zebrafish larvae

• Published in: International journal of nanomedicine Vol. 13; pp. 5377 - 5394
• Main Authors: Zhang, Xiaolin, Song, Jiankang, Klymov, Alexey, Zhang, Yang, de Boer, Leonie, Jansen, John, van den Beucken, Jeroen, Yang, Fang, Zaat, Sebastian, Leeuwenburgh, Sander
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2018; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Additives; Animal genetic engineering; Animals; Antibacterial agents; Antibiotics; Antimicrobial agents; Bacteria; Bacterial infections; bio-distribution; Biological products; Biomedical materials; Care and treatment; cell-material interaction; Drug Delivery Systems; Embryos; Endocytosis - drug effects; Express delivery services; Fluorescence; Fluorescence microscopy; Fluorescent Dyes - metabolism; Gelatin - chemistry; Genetic engineering; Green Fluorescent Proteins - metabolism; Health aspects; Humans; Immune system; in vivo real-time monitoring; Infection; Injections, Intramuscular; Injections, Intravenous; intracellular infection; Larva - cytology; Larva - drug effects; Macrophages; Macrophages - drug effects; Macrophages - metabolism; Medical research; Microscopy; Nanoparticles; Nanospheres - chemistry; Nanospheres - ultrastructure; Original Research; Osteomyelitis; Pathogens; Polymers; Staphylococcal infections; Staphylococcal Infections - drug therapy; Staphylococcal Infections - microbiology; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus infections; Staphylococcus infections; Survival Analysis; Tissue Distribution; Vancomycin; Vancomycin - administration & dosage; Vancomycin - pharmacology; Zebrafish; Zebrafish - metabolism; Zebrafish - microbiology; Netherlands; biodistribution; in vivo real-time monitoring; intracellular infection; cell-material interaction; fluorescence microscopy; Staphylococcus aureus

Infections such as biomaterial-associated infection and osteomyelitis are often associated with intracellular survival of bacteria (eg, ). Treatment of these infections remains a major challenge due to the low intracellular efficacy of many antibiotics. Therefore, local delivery systems are urgently...