Structural Deformation of MTX Induced by Nanodrug Conjugation Dictate Intracellular Drug Transport and Drug Efficacy

Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essentia...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of nanomedicine Vol. 16; pp. 4943 - 4957
Main Authors Park, Jun-Young, Hyun, Ja-Shil, Jee, Jun-Goo, Park, Sung Jean, Khang, Dongwoo
Format Journal Article
LanguageEnglish
Published New Zealand Dove Medical Press Limited 01.01.2021
Taylor & Francis Ltd
Dove
Dove Medical Press
Subjects
Arthritis
carbon nanotube
covalent conjugation
Dihydrofolate reductase
Drug Delivery Systems
Drug dosages
Drug therapy
Drugs
Humans
Methotrexate
Nanoparticles
Nanotubes
Nanotubes, Carbon
Original Research
Pharmaceutical Preparations
rheumatoid arthritis
Rheumatoid factor
structural deformation
Vehicles
South Korea
methotrexate
carbon nanotube
covalent conjugation
rheumatoid arthritis
structural deformation
Online AccessGet full text

Cover

Abstract Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essential to understand the corresponding nanodrug efficacy. The objective of this study is to present an optimal synthesis method for efficient drug delivery through a clear structural analysis of nanodrugs according to the type of conjugation. In this study, the structural variation of methotrexate (MTX) surrounding carbon nanotubes, depending on the type of conjugation style, such as covalent and non-covalent (PEGylation) bonds, was investigated. Specifically, covalent bonds of MTX surrounding CNTs induced greater structural deformation compared to non-covalent bonds (ie, PEGylated CNT). Greater changes in the structural variations of MTX analyzed by nuclear magnetic resonance (NMR) significantly improved the anti-inflammatory drug efficacy of human fibroblast-like synovial cells (FLS) via stable drug release in the extracellular environment and burst drug release under intracellular conditions.
AbstractList Jun-Young Park,1 Ja-Shil Hyun,2 Jun-Goo Jee,3 Sung Jean Park,2 Dongwoo Khang1,4 1Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 21999, Republic of Korea; 2College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, 21936, Republic of Korea; 3Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daegu, 41566, Republic of Korea; 4Department of Physiology, College of Medicine, Gachon University, Incheon, 21999, Republic of KoreaCorrespondence: Dongwoo KhangDepartment of Physiology, College of Medicine, Gachon University, Incheon, 21999, South KoreaTel +82-32-899-6515Email dkhang@gachon.ac.krSung Jean ParkCollege of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, 21936, Republic of KoreaTel +82-32-899-6113Email psjnmr@gachon.ac.krBackground: Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essential to understand the corresponding nanodrug efficacy.Purpose: The objective of this study is to present an optimal synthesis method for efficient drug delivery through a clear structural analysis of nanodrugs according to the type of conjugation.Methods and Results: In this study, the structural variation of methotrexate (MTX) surrounding carbon nanotubes, depending on the type of conjugation style, such as covalent and non-covalent (PEGylation) bonds, was investigated. Specifically, covalent bonds of MTX surrounding CNTs induced greater structural deformation compared to non-covalent bonds (ie, PEGylated CNT).Conclusion: Greater changes in the structural variations of MTX analyzed by nuclear magnetic resonance (NMR) significantly improved the anti-inflammatory drug efficacy of human fibroblast-like synovial cells (FLS) via stable drug release in the extracellular environment and burst drug release under intracellular conditions.Keywords: structural deformation, methotrexate, covalent conjugation, rheumatoid arthritis, carbon nanotube
Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essential to understand the corresponding nanodrug efficacy. The objective of this study is to present an optimal synthesis method for efficient drug delivery through a clear structural analysis of nanodrugs according to the type of conjugation. In this study, the structural variation of methotrexate (MTX) surrounding carbon nanotubes, depending on the type of conjugation style, such as covalent and non-covalent (PEGylation) bonds, was investigated. Specifically, covalent bonds of MTX surrounding CNTs induced greater structural deformation compared to non-covalent bonds (ie, PEGylated CNT). Greater changes in the structural variations of MTX analyzed by nuclear magnetic resonance (NMR) significantly improved the anti-inflammatory drug efficacy of human fibroblast-like synovial cells (FLS) via stable drug release in the extracellular environment and burst drug release under intracellular conditions.
Background: Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essential to understand the corresponding nanodrug efficacy. Purpose: The objective of this study is to present an optimal synthesis method for efficient drug delivery through a clear structural analysis of nanodrugs according to the type of conjugation. Methods and Results: In this study, the structural variation of methotrexate (MTX) surrounding carbon nanotubes, depending on the type of conjugation style, such as covalent and non-covalent (PEGylation) bonds, was investigated. Specifically, covalent bonds of MTX surrounding CNTs induced greater structural deformation compared to non-covalent bonds (ie, PEGylated CNT). Conclusion: Greater changes in the structural variations of MTX analyzed by nuclear magnetic resonance (NMR) significantly improved the anti-inflammatory drug efficacy of human fibroblast-like synovial cells (FLS) via stable drug release in the extracellular environment and burst drug release under intracellular conditions.
Background: Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for increasing nano drug efficacy. In this regard, analyzing the conformational deformation of conjugated drugs surrounding nanoparticles is essential to understand the corresponding nanodrug efficacy. Purpose: The objective of this study is to present an optimal synthesis method for efficient drug delivery through a clear structural analysis of nanodrugs according to the type of conjugation. Methods and Results: In this study, the structural variation of methotrexate (MTX) surrounding carbon nanotubes, depending on the type of conjugation style, such as covalent and non-covalent (PEGylation) bonds, was investigated. Specifically, covalent bonds of MTX surrounding CNTs induced greater structural deformation compared to non-covalent bonds (ie, PEGylated CNT). Conclusion: Greater changes in the structural variations of MTX analyzed by nuclear magnetic resonance (NMR) significantly improved the anti-inflammatory drug efficacy of human fibroblast-like synovial cells (FLS) via stable drug release in the extracellular environment and burst drug release under intracellular conditions. Keywords: structural deformation, methotrexate, covalent conjugation, rheumatoid arthritis, carbon nanotube
Audience Academic
Author Jee, Jun-Goo
Hyun, Ja-Shil
Park, Jun-Young
Khang, Dongwoo
Park, Sung Jean
Author_xml – sequence: 1
  givenname: Jun-Young
  surname: Park
  fullname: Park, Jun-Young
  organization: Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 21999, Republic of Korea
– sequence: 2
  givenname: Ja-Shil
  surname: Hyun
  fullname: Hyun, Ja-Shil
  organization: College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, 21936, Republic of Korea
– sequence: 3
  givenname: Jun-Goo
  surname: Jee
  fullname: Jee, Jun-Goo
  organization: Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daegu, 41566, Republic of Korea
– sequence: 4
  givenname: Sung Jean
  orcidid: 0000-0002-8563-6272
  surname: Park
  fullname: Park, Sung Jean
  organization: College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, Incheon, 21936, Republic of Korea
– sequence: 5
  givenname: Dongwoo
  orcidid: 0000-0003-2353-8017
  surname: Khang
  fullname: Khang, Dongwoo
  organization: Department of Physiology, College of Medicine, Gachon University, Incheon, 21999, Republic of Korea
BackLink https://www.ncbi.nlm.nih.gov/pubmed/34326636$$D View this record in MEDLINE/PubMed
BookMark eNptks-LEzEUxwdZcX_oybsMeJTWSTKTpBdhaVetrOthK3gLb5KXmjJNupmM0P_e1KnrFiSHhJfv-_C-j-9lceaDx6J4TaopJbV4v_xyN71nRFBGnhUXhAg5oRVhZ0_e58Vl32-qqhGSz14U56xmlHPGL4p0n-Kg0xChKxdoQ9xCcsGXwZZfVz_KpTeDRlO2-_IOfDBxWJfz4DfDepQtnE6QMOtSBI1dN3QQy8VBtorg-12IqQRvxtKNtU6D3r8snlvoenx1vK-K7x9vVvPPk9tvn5bz69uJbmqaJo2UEgwYjoa2eVqkLWIjjSBCo0XLBamgqpBZQERrW7Si0W2r60Zbrhm7KpYj1wTYqF10W4h7FcCpP4UQ1wpicrpDVWnJCCcA9czUummkMK1pScvreiYokZn1YWTthnaLRuPBcXcCPf3x7qdah18qcxsqZxnw9giI4WHAPqlNGKLP_hVtOK0Ym1H5T7WGPJXzNhwWu3W9VtdcUF4LSQ7Gpv9R5WNw63SOh3W5ftLwbmzQMfR9RPs4OKnUIUUqp0gdU5TVb556fdT-jQ37DRcPxcI
CitedBy_id crossref_primary_10_2147_IJN_S383324
crossref_primary_10_1016_j_ijpharm_2023_122698
crossref_primary_10_58502_DTT_23_0013
crossref_primary_10_2147_IJN_S436372
Cites_doi 10.3389/fbioe.2020.00127
10.3390/pharmaceutics12060592
10.1038/s41397-018-0017-5
10.1021/acs.accounts.8b00307
10.1039/C9NR04323C
10.3390/pharmaceutics11070325
10.1186/s12896-016-0262-0
10.1016/j.addr.2019.04.008
10.3390/ma12182889
10.1007/s10565-009-9122-1
10.1021/ja052074m
10.1155/2020/1537689
10.1186/1477-3155-11-26
10.1039/D0TB02527E
10.1021/nn4041206
10.1039/D0PY90048F
10.1039/C8TB02302F
10.1111/bcp.14057
10.2147/IJN.S142122
10.1016/j.msec.2013.11.046
10.1186/s11671-019-3046-3
10.1016/j.ejmech.2008.05.031
10.1006/jmre.1998.1519
10.1007/BF02192855
10.1002/art.37932
10.1002/advs.202004979
10.1021/acsmacrolett.5b00935
10.1016/j.canlet.2021.01.026
10.1038/nprot.2009.146
10.1021/acsnano.9b03824
10.5402/2012/637094
10.1039/D0BM00276C
10.1177/1759720X11408635
10.1002/jcp.27860
10.1166/jnn.2015.10614
10.1126/science.1222453
10.2147/IJN.S254808
10.1186/s40824-019-0166-x
10.2147/IJN.S263238
10.1038/s41598-017-06796-7
10.1021/acsami.9b03983
10.3390/pharmaceutics13040453
ContentType Journal Article
Copyright 2021 Park et al.
COPYRIGHT 2021 Dove Medical Press Limited
2021. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
2021 Park et al. 2021 Park et al.
Copyright_xml – notice: 2021 Park et al.
– notice: COPYRIGHT 2021 Dove Medical Press Limited
– notice: 2021. This work is licensed under https://creativecommons.org/licenses/by-nc/3.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.
– notice: 2021 Park et al. 2021 Park et al.
DBID CGR
CUY
CVF
ECM
EIF
NPM
AAYXX
CITATION
3V.
7X7
7XB
8FI
8FJ
8FK
8G5
ABUWG
AFKRA
AZQEC
BENPR
CCPQU
DWQXO
FYUFA
GHDGH
GNUQQ
GUQSH
K9.
M0S
M2O
MBDVC
PIMPY
PQEST
PQQKQ
PQUKI
PRINS
Q9U
5PM
DOI 10.2147/IJN.S317231
DatabaseName Medline
MEDLINE
MEDLINE (Ovid)
MEDLINE
MEDLINE
PubMed
CrossRef
ProQuest Central (Corporate)
ProQuest Health & Medical Collection
ProQuest Central (purchase pre-March 2016)
Hospital Premium Collection
Hospital Premium Collection (Alumni Edition)
ProQuest Central (Alumni) (purchase pre-March 2016)
Research Library (Alumni Edition)
ProQuest Central (Alumni)
ProQuest Central
ProQuest Central Essentials
AUTh Library subscriptions: ProQuest Central
ProQuest One Community College
ProQuest Central
Health Research Premium Collection
Health Research Premium Collection (Alumni)
ProQuest Central Student
Research Library Prep
ProQuest Health & Medical Complete (Alumni)
Health & Medical Collection (Alumni Edition)
ProQuest research library
Research Library (Corporate)
Publicly Available Content Database
ProQuest One Academic Eastern Edition (DO NOT USE)
ProQuest One Academic
ProQuest One Academic UKI Edition
ProQuest Central China
ProQuest Central Basic
PubMed Central (Full Participant titles)
DatabaseTitle MEDLINE
Medline Complete
MEDLINE with Full Text
PubMed
MEDLINE (Ovid)
CrossRef
Publicly Available Content Database
Research Library Prep
ProQuest Central Student
ProQuest Central Basic
ProQuest Central Essentials
ProQuest One Academic Eastern Edition
ProQuest Health & Medical Complete (Alumni)
ProQuest Central (Alumni Edition)
ProQuest One Community College
ProQuest Hospital Collection
Research Library (Alumni Edition)
Health Research Premium Collection (Alumni)
ProQuest Central China
ProQuest Hospital Collection (Alumni)
ProQuest Central
ProQuest Health & Medical Complete
Health Research Premium Collection
ProQuest One Academic UKI Edition
Health and Medicine Complete (Alumni Edition)
ProQuest Central Korea
ProQuest Research Library
ProQuest One Academic
ProQuest Central (Alumni)
DatabaseTitleList
MEDLINE
Publicly Available Content Database
Database_xml – sequence: 1
  dbid: NPM
  name: PubMed
  url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed
  sourceTypes: Index Database
– sequence: 2
  dbid: EIF
  name: MEDLINE
  url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search
  sourceTypes: Index Database
– sequence: 3
  dbid: BENPR
  name: AUTh Library subscriptions: ProQuest Central
  url: https://www.proquest.com/central
  sourceTypes: Aggregation Database
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
DocumentTitleAlternate Park et al
EISSN 1178-2013
EndPage 4957
ExternalDocumentID oai_doaj_org_article_0c83161aa49d4c5587dbdb1b64497218
A672647813
10_2147_IJN_S317231
34326636
Genre Journal Article
GeographicLocations South Korea
GeographicLocations_xml – name: South Korea
GroupedDBID ---
0YH
29J
2WC
53G
5GY
5VS
7X7
8FI
8FJ
8G5
ABUWG
ACGFO
ADBBV
ADRAZ
AEGXH
AENEX
AFKRA
AIAGR
ALIPV
ALMA_UNASSIGNED_HOLDINGS
AOIJS
AZQEC
BAWUL
BCNDV
BENPR
BPHCQ
BVXVI
CCPQU
CGR
CS3
CUY
CVF
DIK
DU5
DWQXO
E3Z
EBD
EBS
ECM
EIF
EJD
EMOBN
F5P
FYUFA
GNUQQ
GROUPED_DOAJ
GUQSH
GX1
HMCUK
HYE
IAO
IHR
ITC
KQ8
M2O
M48
MM.
M~E
NPM
O5R
O5S
OK1
P2P
PGMZT
PIMPY
PQQKQ
PROAC
RNS
RPM
SJN
SV3
TR2
UKHRP
VDV
AAYXX
CITATION
TDBHL
3V.
7XB
8FK
K9.
MBDVC
PQEST
PQUKI
PRINS
Q9U
5PM
ID FETCH-LOGICAL-c542t-5888adad6ed2b636e2bee58d717cefef6710a00e3faeeeffbef75cbbc45cf6c33
IEDL.DBID RPM
ISSN 1178-2013
1176-9114
IngestDate Thu Sep 05 15:34:32 EDT 2024
Tue Sep 17 21:14:43 EDT 2024
Wed Sep 25 01:15:05 EDT 2024
Thu Feb 22 23:42:25 EST 2024
Tue Aug 27 01:21:52 EDT 2024
Thu Sep 26 18:30:20 EDT 2024
Sat Sep 28 08:11:16 EDT 2024
IsDoiOpenAccess true
IsOpenAccess true
IsPeerReviewed true
IsScholarly true
Keywords methotrexate
carbon nanotube
covalent conjugation
rheumatoid arthritis
structural deformation
Language English
License 2021 Park et al.
This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
LinkModel DirectLink
MergedId FETCHMERGED-LOGICAL-c542t-5888adad6ed2b636e2bee58d717cefef6710a00e3faeeeffbef75cbbc45cf6c33
ORCID 0000-0003-2353-8017
0000-0002-8563-6272
OpenAccessLink https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8315289/
PMID 34326636
PQID 2562033928
PQPubID 3933144
PageCount 15
ParticipantIDs doaj_primary_oai_doaj_org_article_0c83161aa49d4c5587dbdb1b64497218
pubmedcentral_primary_oai_pubmedcentral_nih_gov_8315289
proquest_journals_2562033928
gale_infotracmisc_A672647813
gale_infotracacademiconefile_A672647813
crossref_primary_10_2147_IJN_S317231
pubmed_primary_34326636
PublicationCentury 2000
PublicationDate 2021-01-01
PublicationDateYYYYMMDD 2021-01-01
PublicationDate_xml – month: 01
  year: 2021
  text: 2021-01-01
  day: 01
PublicationDecade 2020
PublicationPlace New Zealand
PublicationPlace_xml – name: New Zealand
– name: Macclesfield
PublicationTitle International journal of nanomedicine
PublicationTitleAlternate Int J Nanomedicine
PublicationYear 2021
Publisher Dove Medical Press Limited
Taylor & Francis Ltd
Dove
Dove Medical Press
Publisher_xml – name: Dove Medical Press Limited
– name: Taylor & Francis Ltd
– name: Dove
– name: Dove Medical Press
References Francia (ref42) 2019; 13
Lee (ref44) 2017; 12
De Volder (ref2) 2013; 339
Hao (ref5) 2020; 11
Wang (ref21) 2020; 2020
Cheng (ref35) 2009; 44
Schulte (ref31) 1998; 134
Muthukumar (ref29) 2014; 36
Kim (ref30) 2017; 7
Yadav (ref8) 2020; 8
Choy (ref20) 2017; 56
(ref34) 2015
Broeren (ref33) 2005; 127
Fawal (ref15) 2021; 503
Affleck (ref39) 2010; 26
Braun (ref19) 2011; 3
Li (ref11) 2020; 8
Chakrabarti (ref4) 2015; 15
Rajora (ref12) 2020; 12
Park (ref13) 2020; 15
Skubisz (ref14) 2012; 2012
Lopez-Rodriguez (ref17) 2018; 18
Chen (ref23) 2019; 7
Chenthamara (ref24) 2019; 23
Que (ref10) 2016; 5
Nam (ref36) 2013; 65
Liu (ref7) 2009; 4
Abbasi (ref22) 2019; 234
Tran (ref6) 2019; 11
Venkataraman (ref26) 2019; 14
Lee (ref40) 2013; 7
Cirillo (ref27) 2019; 12
Chai (ref16) 2019; 11
Saptarshi (ref41) 2013; 11
Feng (ref9) 2018; 51
Lucas (ref18) 2019; 85
Donahue (ref1) 2019; 143
Kofoed Andersen (ref28) 2021; 13
Lee (ref37) 2019; 11
Aswad (ref38) 2016; 16
Piotto (ref32) 1992; 2
Sheikhpour (ref25) 2020; 15
Park (ref43) 2021; 8
Liu (ref3) 2021; 9
References_xml – volume: 8
  start-page: 127
  year: 2020
  ident: ref8
  publication-title: Front Bioeng Biotechnol
  doi: 10.3389/fbioe.2020.00127
  contributor:
    fullname: Yadav
– volume: 12
  start-page: 592
  year: 2020
  ident: ref12
  publication-title: Pharmaceutics
  doi: 10.3390/pharmaceutics12060592
  contributor:
    fullname: Rajora
– volume: 18
  start-page: 539
  year: 2018
  ident: ref17
  publication-title: Pharmacogenomics J
  doi: 10.1038/s41397-018-0017-5
  contributor:
    fullname: Lopez-Rodriguez
– volume: 51
  start-page: 2314
  year: 2018
  ident: ref9
  publication-title: Acc Chem Res
  doi: 10.1021/acs.accounts.8b00307
  contributor:
    fullname: Feng
– volume: 11
  start-page: 19980
  year: 2019
  ident: ref37
  publication-title: Nanoscale
  doi: 10.1039/C9NR04323C
  contributor:
    fullname: Lee
– volume: 11
  start-page: 325
  year: 2019
  ident: ref6
  publication-title: Pharmaceutics
  doi: 10.3390/pharmaceutics11070325
  contributor:
    fullname: Tran
– volume: 16
  start-page: 32
  year: 2016
  ident: ref38
  publication-title: BMC Biotechnol
  doi: 10.1186/s12896-016-0262-0
  contributor:
    fullname: Aswad
– volume: 143
  start-page: 68
  year: 2019
  ident: ref1
  publication-title: Adv Drug Deliv Rev
  doi: 10.1016/j.addr.2019.04.008
  contributor:
    fullname: Donahue
– volume: 12
  start-page: 2889
  year: 2019
  ident: ref27
  publication-title: Materials (Basel)
  doi: 10.3390/ma12182889
  contributor:
    fullname: Cirillo
– volume: 26
  start-page: 117
  year: 2010
  ident: ref39
  publication-title: Cell Biol Toxicol
  doi: 10.1007/s10565-009-9122-1
  contributor:
    fullname: Affleck
– volume: 127
  start-page: 10334
  year: 2005
  ident: ref33
  publication-title: J Am Chem Soc
  doi: 10.1021/ja052074m
  contributor:
    fullname: Broeren
– volume: 2020
  start-page: 1537689
  year: 2020
  ident: ref21
  publication-title: J Ophthalmol
  doi: 10.1155/2020/1537689
  contributor:
    fullname: Wang
– volume: 11
  start-page: 26
  year: 2013
  ident: ref41
  publication-title: J Nanobiotechnology
  doi: 10.1186/1477-3155-11-26
  contributor:
    fullname: Saptarshi
– volume: 9
  start-page: 952
  year: 2021
  ident: ref3
  publication-title: J Mater Chem B
  doi: 10.1039/D0TB02527E
  contributor:
    fullname: Liu
– volume: 7
  start-page: 8484
  year: 2013
  ident: ref40
  publication-title: ACS Nano
  doi: 10.1021/nn4041206
  contributor:
    fullname: Lee
– volume: 11
  start-page: 2923
  year: 2020
  ident: ref5
  publication-title: Polym Chem
  doi: 10.1039/D0PY90048F
  contributor:
    fullname: Hao
– volume: 7
  start-page: 112
  year: 2019
  ident: ref23
  publication-title: J Mater Chem B
  doi: 10.1039/C8TB02302F
  contributor:
    fullname: Chen
– volume: 85
  start-page: 2228
  year: 2019
  ident: ref18
  publication-title: Br J Clin Pharmacol
  doi: 10.1111/bcp.14057
  contributor:
    fullname: Lucas
– volume: 12
  start-page: 5761
  year: 2017
  ident: ref44
  publication-title: Int J Nanomedicine
  doi: 10.2147/IJN.S142122
  contributor:
    fullname: Lee
– volume: 36
  start-page: 14
  year: 2014
  ident: ref29
  publication-title: Mater Sci Eng C Mater Biol Appl
  doi: 10.1016/j.msec.2013.11.046
  contributor:
    fullname: Muthukumar
– volume: 14
  start-page: 220
  year: 2019
  ident: ref26
  publication-title: Nanoscale Res Lett
  doi: 10.1186/s11671-019-3046-3
  contributor:
    fullname: Venkataraman
– volume: 44
  start-page: 2219
  year: 2009
  ident: ref35
  publication-title: Eur J Med Chem
  doi: 10.1016/j.ejmech.2008.05.031
  contributor:
    fullname: Cheng
– volume: 134
  start-page: 168
  year: 1998
  ident: ref31
  publication-title: J Magn Reson
  doi: 10.1006/jmre.1998.1519
  contributor:
    fullname: Schulte
– volume: 2
  start-page: 661
  year: 1992
  ident: ref32
  publication-title: J Biomol NMR
  doi: 10.1007/BF02192855
  contributor:
    fullname: Piotto
– volume: 65
  start-page: 1753
  year: 2013
  ident: ref36
  publication-title: Arthritis Rheum
  doi: 10.1002/art.37932
  contributor:
    fullname: Nam
– volume: 8
  start-page: 2004979
  year: 2021
  ident: ref43
  publication-title: Adv Sci (Weinh)
  doi: 10.1002/advs.202004979
  contributor:
    fullname: Park
– volume: 5
  start-page: 168
  year: 2016
  ident: ref10
  publication-title: ACS Macro Lett
  doi: 10.1021/acsmacrolett.5b00935
  contributor:
    fullname: Que
– volume: 503
  start-page: 129
  year: 2021
  ident: ref15
  publication-title: Cancer Lett
  doi: 10.1016/j.canlet.2021.01.026
  contributor:
    fullname: Fawal
– volume: 4
  start-page: 1372
  year: 2009
  ident: ref7
  publication-title: Nat Protoc
  doi: 10.1038/nprot.2009.146
  contributor:
    fullname: Liu
– volume: 13
  start-page: 11107
  year: 2019
  ident: ref42
  publication-title: ACS Nano
  doi: 10.1021/acsnano.9b03824
  contributor:
    fullname: Francia
– volume: 2012
  start-page: 637094
  year: 2012
  ident: ref14
  publication-title: ISRN Obstet Gynecol
  doi: 10.5402/2012/637094
  contributor:
    fullname: Skubisz
– volume: 8
  start-page: 2300
  year: 2020
  ident: ref11
  publication-title: Biomateri Sci
  doi: 10.1039/D0BM00276C
  contributor:
    fullname: Li
– volume: 3
  start-page: 151
  year: 2011
  ident: ref19
  publication-title: Ther Adv Musculoskelet Dis
  doi: 10.1177/1759720X11408635
  contributor:
    fullname: Braun
– volume: 234
  start-page: 10018
  year: 2019
  ident: ref22
  publication-title: J Cell Physiol
  doi: 10.1002/jcp.27860
  contributor:
    fullname: Abbasi
– volume: 15
  start-page: 5501
  year: 2015
  ident: ref4
  publication-title: J Nanosci Nanotechnol
  doi: 10.1166/jnn.2015.10614
  contributor:
    fullname: Chakrabarti
– volume: 339
  start-page: 535
  year: 2013
  ident: ref2
  publication-title: Science
  doi: 10.1126/science.1222453
  contributor:
    fullname: De Volder
– volume: 15
  start-page: 5783
  year: 2020
  ident: ref13
  publication-title: Int J Nanomedicine
  doi: 10.2147/IJN.S254808
  contributor:
    fullname: Park
– volume: 56
  start-page: 689
  year: 2017
  ident: ref20
  publication-title: Rheumatology (Oxford)
  contributor:
    fullname: Choy
– volume: 23
  start-page: 20
  year: 2019
  ident: ref24
  publication-title: Biomater Res
  doi: 10.1186/s40824-019-0166-x
  contributor:
    fullname: Chenthamara
– volume: 15
  start-page: 7063
  year: 2020
  ident: ref25
  publication-title: Int J Nanomedicine
  doi: 10.2147/IJN.S263238
  contributor:
    fullname: Sheikhpour
– volume: 7
  start-page: 6454
  year: 2017
  ident: ref30
  publication-title: Sci Rep
  doi: 10.1038/s41598-017-06796-7
  contributor:
    fullname: Kim
– volume-title: AMBER 2015
  year: 2015
  ident: ref34
– volume: 11
  start-page: 22915
  year: 2019
  ident: ref16
  publication-title: ACS Appl Mater Interfaces
  doi: 10.1021/acsami.9b03983
  contributor:
    fullname: Chai
– volume: 13
  start-page: 453
  year: 2021
  ident: ref28
  publication-title: Pharmaceutics
  doi: 10.3390/pharmaceutics13040453
  contributor:
    fullname: Kofoed Andersen
SSID ssj0057869
Score 2.3649232
Snippet Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport and for...
Background: Understanding structural interactions between the active drug and conjugated nanoparticles is critical for optimizing intracellular drug transport...
Jun-Young Park,1 Ja-Shil Hyun,2 Jun-Goo Jee,3 Sung Jean Park,2 Dongwoo Khang1,4 1Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon, 21999,...
SourceID doaj
pubmedcentral
proquest
gale
crossref
pubmed
SourceType Open Website
Open Access Repository
Aggregation Database
Index Database
StartPage 4943
SubjectTerms Arthritis
carbon nanotube
covalent conjugation
Dihydrofolate reductase
Drug Delivery Systems
Drug dosages
Drug therapy
Drugs
Humans
Methotrexate
Nanoparticles
Nanotubes
Nanotubes, Carbon
Original Research
Pharmaceutical Preparations
rheumatoid arthritis
Rheumatoid factor
structural deformation
Vehicles
SummonAdditionalLinks – databaseName: DOAJ Directory of Open Access Journals
  dbid: DOA
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV07b9wwDBaKTM1Q9JG2btNCQ4BObmzJkn1jmkuQBkiWJMBtgkRRaTr4gvYy5N-XtHyHMzJ0yWoR0IMfRdIQPwpxEKOmMN2bkhKgUDaooPRgUxlUSuhNHSvgAueLS3t205wvzGKr1Re_Ccv0wPngDivoNEUl3jez2IAxXRtDDHUgP87EM7nMtzbrZCrfwQTDoZldXbeMg1rnyjzuyXP48_zy-xV5TaXriS8aKPufXsxbnmn6anLLDZ2-Fq_G-FEe5XW_ES-wfyt2t1gF34nV1cAJy3waco6b6kS5TPLieiG5WQdglOFR0tW6jH8ebuXxsv_9cJvF5nfAASjJ0fz8X58fqso5i22Y0KXvY_50whQUHh73xM3pyfXxWTk2VyjBNGpVGkp9ffTRYlTBaosqIJouUnoHmDBZCj18VaFOHhFTCphaAyFAYyBZ0Pq92OmXPX4UkszeGxOr1EFoou06oCylCpUOMz-bJSjEwfqY3X3m0HCUe7A2HGnDjdooxA9WwUaEia-HDwQHN8LB_Q8OhfjGCnRsnnxKfqwyoJUy0ZU7sq0aymt1IfYnkmRWMB1eQ8CNZv3XUXyoKk0hJc3zIaNhs1yu0KXwzRaineBksp_pSH_3ayD0pi0ZSnw_PccBfBYvFT-7Gf4S7YsdQhx-obhpFb4OJvIPceYXIg
  priority: 102
  providerName: Directory of Open Access Journals
– databaseName: ProQuest Health & Medical Collection
  dbid: 7X7
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1LT9wwELYovbSHqvQZSisfkHpKN7FjJ3uqgAVtkeACSHuz7LG9pUIJheXAv-9Mkg0bVeKajJTE883L8XzD2L73EtN0q1IsgFxaBAGpBR1TJ2IMVuU-A2pwPjvX86vidKEWW2y-7oWhY5Vrn9g6at8A7ZFPMDSLTGI0rybW0S4ArCY_b_-mND-K_rP2wzResJe5wLQCkV0uhtILYdkOt8vzUpN5F12nHs3omfw6Pf9xgVFUyHwUm1oK__8d9UakGp-i3AhLJ2_Zmz6f5AcdAHbYVqjfsdcbLIPv2eqi5Yglfg0-C0O3Im8iP7tccBreAcFz98jR1Tb-7mHJj5r6z8OyE5tdAyWkKEfLEW5u6OAqn5HYwIzObe27S8dESWHh8QO7Ojm-PJqn_bCFFFQhVqnCUth663Xwwmmpg3AhqMpjuQchhqgxFbFZFmS0IYQYXYilAuegUBA1SPmRbddNHT4zjm7AKuWzWIErvK4qwKolc5l0UzudRkjY_nqZzW3HqWGwFiFtGNSG6bWRsENSwSBCRNjtheZuaXq7MhlUEpNWa4upL0CpqvTOu9xhmke8RFXCvpMCDZkrrZLtuw7wTYn4yhzoUrTttjJheyNJNDMY315DwPRmfm-eQJmwTx0ahteljl1M53TCyhFORt8zvlNf_24JvvGTFBbCu88_8gt7JeiATbsftMe2EUvhK2ZIK_etBf8_wygTBw
  priority: 102
  providerName: ProQuest
– databaseName: Scholars Portal Open Access Journals
  dbid: M48
  link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwdV1La9wwEBYhgZIeSt91mxYdAj059epl-xBCmk1IA5tLsrA3oec2pdjtdgPdf58Zv1j3cbXHL80nzTey5hMhh95zoOlGppAA2VQE5lLjVEwtizEYOfGZwwLn2bW6nIurhVzskH4zzq4Bf_0ztcP9pOar70e_f25OoMMf4zLmicg_fbm6PrqBOMiwnnqPCS4Q6jMx_E4AVKqyLc7784J98ghLKyHuqlFkagT8_x6mt-LUeA3lVlC6eEqedGySnrbuf0Z2QvWcPN7SGHxB1jeNQiyqa9BpGGoVaR3p7HZBcesOFzy1GwoDbe1X90t6Vlff7pet2fTOIR0FO3g-zvLjslU6RbNBF52ayreHzlGQwrjNSzK_OL89u0y7rRZSJwVbpxISYeONV8EzC20RmA1BFh6SPRdiiAqIiMmywKMJIcRoQ8yls9YJ6aJynL8iu1VdhTeEwiBgpPRZLJwVXhWFg5wlsxm3pSnL6BJy2Dez_tEqamjIRNAxGhyjO8ck5DO6YDBBGezmQL1a6q5X6cwVHCirMaL0wklZ5N56O7FA8lCVqEjIR3SgRvhgK5mu5gDeFGWv9KnKWVNsyxNyMLKETubGp3sI6B6jGtgiyzgQTHjO6xYNw-v2oEpIPsLJ6HvGZ6q7r428N3yShDT47X_v-Y7sM1xZ00wEHZBdgFF4D9RobT80sH8Ah4APQg
  priority: 102
  providerName: Scholars Portal
Title Structural Deformation of MTX Induced by Nanodrug Conjugation Dictate Intracellular Drug Transport and Drug Efficacy
URI https://www.ncbi.nlm.nih.gov/pubmed/34326636
https://www.proquest.com/docview/2562033928/abstract/
https://pubmed.ncbi.nlm.nih.gov/PMC8315289
https://doaj.org/article/0c83161aa49d4c5587dbdb1b64497218
Volume 16
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9swDCaaDhjaw7B3vXWBDgV2cmJblmwf2yRFViBBsbZAdjL0zDKsdtGlh_77UbIdxNhtFx9sGpbFjyIpkJ8AzrSmGKYLFmICJMPUJCoUittQJtYawWIdKdfgvFjy-V16tWKrA2BdL4wv2ldyM6p-34-qzU9fW_lwr8Zdndj4ejHJKXqdvBgPYJBR2qXozfKLCPTn2MVxxp0lp01TnjuOZ_ztajm6QYeJMc0RvHQtlehvec8jeeL-f5fnPf_Ur53cc0aXr-FVG0WS82a0b-DAVG_heI9b8B1sbzwzrGPVIFOz61EktSWL2xVxR3Yoo4l8JrjA1vrxaU0mdfXrad2ITTfKhaEoh993u_uuXJVMndiOD52ISje3Zo6IQqjn93B3ObudzMP2iIVQsTTZhgwTYKGF5kYnEufCJNIYlmtM8pSxxnIMQEQUGWqFMcZaaWzGlJQqZcpyRekHOKzqypwAQeMXjOnI5kqmmue5wlwlkhGVhSgKqwI466a5fGiYNErMQJxiSlRM2SomgAungp2Io7_2N-rHddmCoIwUQoDHQqSFThVjeaallrHE4M6xEeUBfHUKLJ2RulkSba8BjtTRXZXnPEt8ky0N4LQnical-o87CJStcf8pMUpMIoqBJX7nY4OG3XA7UAWQ9XDS-5_-E0S5p_VuUf3pv9_8DEeJq7jxG0SncIgwM18wZNrKIQyiH3O8ZqtsCC8uZsvr70O__YDXRZoPvQn9BcL1HZU
link.rule.ids 230,315,733,786,790,870,891,2115,12083,21416,24346,27957,27958,31754,33779,43345,43840,53827,53829,74102,74659
linkProvider National Library of Medicine
linkToHtml http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9swDBa27rDtMOw9r92mQ4GdvNrWw85p6JoWadfk0hTITZAoKesOdtemh_77kbaTxhiwq0RAkkmKpEx-ZGzfe4FuulUpBkAulaGA1IKOqStiDFblPgMqcJ7O9ORSni3Uon9wu-3TKtd3YntR-wbojfwATXORCbTm1ffrPyl1jaK_q30LjcfsiRRCUkpfudgEXCiMbUu7PC81KbXs6vOoM8_B6dns2wXazkLkA4vUAvf_ez1v2adh7uSWMTp5yV70XiQ_7Nj-ij0K9Wv2fAtb8A1bXbTIsISqwcdhU6PIm8in8wWnlh0QPHf3HC_Yxt_cLflRU_--W3Zk4ysgNxTpcH163ad0VT4msg0eOre174aOCYjCwv1bdnlyPD-apH2LhRSULFapwgDYeut18IXTQofChaAqj0EehBiiRgfEZlkQ0YYQYnQhlgqcA6kgahDiHdupmzp8YByV3yrls1iBk15XFWCskrlMuJEdjSIkbH_9mc11h6RhMAIhbhjkhum5kbAfxIINCcFftwPNzdL02mQyqAS6qtbKkZegVFV6513u0LkjNKIqYV-JgYaUlL6S7WsNcKcEd2UOdVm0RbYiYXsDSlQuGE6vRcD0yn1rHkQxYe87adhsl-p00YnTCSsHcjI4z3CmvvrVwnrjkRSGvx__v-QX9nQyn56b89PZz132rKAUm_ZFaI_toFyFT-gjrdznVhH-AtchEjo
linkToPdf http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV1Lb9NAEB5BKyF6QLwxFNhDJU4mjte7dk6obRK1hUYVbaXcVvsM5WD3kR7675mxNyEWEtf1SOv1vNcz3wDsOccxTNcixQTIpIXPbaqtDKnJQ_BaDF1mqcH5dCaPLouTuZjH-qe7WFa5somtoXaNpTvyAbrmPOPozatBiGURZ-Ppt-ublCZI0Z_WOE7jMWyXhRQo4dsHk9nZz5VdRtFsB9wNh6UkFS-6bj2a0zM4Ppl9PUdPmvNhzz-1MP7_GusNb9WvpNxwTdPn8CzGlGy_E4IX8MjXL2FnA2nwFSzPW5xYwthgY7_uWGRNYKcXc0YDPKx3zDwwNLeNu71fsMOm_n2_6MjGV5aCUqTD_emun4pX2ZjI1ujoTNeuW5oQLIW2D6_hcjq5ODxK48CF1IoiX6YC02HttJPe5UZy6XPjvagcpnzWBx8khiM6yzwP2nsfgvGhFNYYWwgbpOX8DWzVTe3fAUNToIVwWaisKZysKouZS2YybkZ6NAo2gb3VZ1bXHa6GwnyEuKGQGypyI4EDYsGahMCw24XmdqGibqnMVhwDV62LkSusEFXpjDNDg6EeYRNVCXwhBipSWfpKOnYe4JsS-JXal2XettzyBHZ7lKhqtv94JQIqqvqd-iuYCbztpGH9utS1iyGdTKDsyUnvPP0n9dWvFuQbjyQwGX7__y0_wxPUAvXjePb9AzzNqd6mvR7ahS0UK_8RA6al-RQ14Q9dhRfd
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Structural+Deformation+of+MTX+Induced+by+Nanodrug+Conjugation+Dictate+Intracellular+Drug+Transport+and+Drug+Efficacy&rft.jtitle=International+journal+of+nanomedicine&rft.au=Park%2C+Jun-Young&rft.au=Hyun%2C+Ja-Shil&rft.au=Jee%2C+Jun-Goo&rft.au=Park%2C+Sung+Jean&rft.date=2021-01-01&rft.eissn=1178-2013&rft.volume=16&rft.spage=4943&rft_id=info:doi/10.2147%2FIJN.S317231&rft_id=info%3Apmid%2F34326636&rft.externalDocID=34326636
thumbnail_l http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1178-2013&client=summon
thumbnail_m http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1178-2013&client=summon
thumbnail_s http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1178-2013&client=summon