Establishment of the first World Health Organization International Genetic Reference Panel for quantitation of BCR-ABL mRNA

• Published in: Blood Vol. 116; no. 22; pp. e111 - e117
• Main Authors: White, Helen E., Matejtschuk, Paul, Rigsby, Peter, Gabert, Jean, Lin, Feng, Lynn Wang, Y., Branford, Susan, Müller, Martin C., Beaufils, Nathalie, Beillard, Emmanuel, Colomer, Dolors, Dvorakova, Dana, Ehrencrona, Hans, Goh, Hyun-Gyung, El Housni, Hakim, Jones, Dan, Kairisto, Veli, Kamel-Reid, Suzanne, Kim, Dong-Wook, Langabeer, Stephen, Ma, Edmond S.K., Press, Richard D., Romeo, Giuliana, Wang, Lihui, Zoi, Katerina, Hughes, Timothy, Saglio, Giuseppe, Hochhaus, Andreas, Goldman, John M., Metcalfe, Paul, Cross, Nicholas C.P.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 25.11.2010
• Subjects: Abl protein; Acute lymphatic leukemia; BCR protein; Blood; Cell Line; Chromosome translocations; Chronic myeloid leukemia; Fusion protein; Fusion Proteins, bcr-abl - genetics; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive - genetics; MEDICIN; MEDICINE; mRNA; Polymerase chain reaction; Quantitation; Reference Standards; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - standards; RNA, Messenger - genetics; Standardization; World Health Organization

Serial quantitation of BCR-ABL mRNA levels is an important indicator of therapeutic response for patients with chronic myelogenous leukemia and Philadelphia chromosome–positive acute lymphoblastic leukemia, but there is substantial variation in the real-time quantitative polymerase chain reaction me...