Interdental Plaque Microbial Community Changes under In Vitro Violet LED Irradiation

• Published in: Antibiotics (Basel) Vol. 10; no. 11; p. 1348
• Main Authors: Wang, Dan, Nambu, Takayuki, Tanimoto, Hiroaki, Iwata, Naohiro, Yoshikawa, Kazushi, Okinaga, Toshinori, Yamamoto, Kazuyo
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 04.11.2021; MDPI
• Subjects: 16S rRNA gene; Antibiotic resistance; Antibiotics; Bacteria; Biofilms; Campylobacter; Dental plaque; Discriminant analysis; Dysbacteriosis; Gene sequencing; Genetic testing; high-throughput sequencing; Irradiation; Light effects; Light emitting diodes; Light irradiation; Microbiomes; Microbiota; Microorganisms; Next-generation sequencing; oral microbiota; Photodynamic therapy; Pneumonia; Relative abundance; rRNA 16S; Systemic diseases; Taxonomy; violet light
• ISSN: 2079-6382; 2079-6382
• DOI: 10.3390/antibiotics10111348

Oral microbiome dysbiosis has important links to human health and disease. Although photodynamic therapy influences microbiome diversity, the specific effect of violet light irradiation remains largely unknown. In this study, we analyzed the effect of violet light-emitting diode (LED) irradiation on interdental plaque microbiota. Interdental plaque was collected from 12 human subjects, exposed to violet LED irradiation, and cultured in a specialized growth medium. Next-generation sequencing of the 16S ribosomal RNA genes revealed that α-diversity decreased, whereas β-diversity exhibited a continuous change with violet LED irradiation doses. In addition, we identified several operational taxonomic units that exhibited significant shifts during violet LED irradiation. Specifically, violet LED irradiation led to a significant reduction in the relative abundance of Fusobacterium species, but a significant increase in several species of oral bacteria, such as Veillonella and Campylobacter. Our study provides an overview of oral plaque microbiota changes under violet LED irradiation, and highlights the potential of this method for adjusting the balance of the oral microbiome without inducing antibiotic resistance.